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Human PB Pan Monocytes (Age: 51), Negative selected, 5 x 10^7 cells (MTS-1022-JF85)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. Monocytes provide an ideal model for the study of macrophage biology and mechanisms.
These pan monocytes are purified from peripheral blood by removing other cell types using immunomagnetic negative selection, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Isolation Method

Negative selection

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

For a mid-age donor (51), will these monocytes still differentiate reliably into macrophages?

Yes. Primary monocytes from adult donors are widely used for macrophage differentiation. The key is controlling the process variables (thaw density, recovery time, cytokine dosing, and media consistency). If you're comparing multiple conditions (M1/M2 polarization, drug treatment), we recommend using the same lot across the entire series and including a small "reference arm" (e.g., M-CSF only) in every batch to normalize drift.

Can I use these cells directly for cytokine profiling or do I need a resting period?

For functional readouts, we strongly recommend a short recovery/resting window post-thaw (often overnight) to reduce stress-response artifacts and improve interpretability. After recovery, you can proceed to stimulation (LPS/IFN-γ, IL-4/IL-13, immune complexes, etc.) with tighter baseline noise. We can also recommend plating formats and density ranges based on your readout (ELISA, qPCR, flow, multiplex).

I need 5×10^7 cells for a large assay-can you advise on plate planning and expected recovery?

Certainly. Large-format work benefits from a plate map that anticipates realistic post-thaw recovery (viability loss + adherence selection). Share your assay format (96/384-well, transwells, co-culture), target replicate number, and endpoints. We'll help you translate "cells per vial" into a practical seeding plan with buffer for QC and pilot optimization, so you don't run short mid-study.

This product offers a dependable foundation and supports stronger experimental conclusions
We used this age 51 donor monocyte batch for comparative studies between different macrophage differentiation media. The performance was consistent and the morphology was uniform across conditions, which makes protocol comparisons meaningful. The cells also produced strong RNA yields for downstream gene expression analysis and tolerated routine handling without excessive clumping. Ordering was straightforward and delivery was reliable.
These monocytes were used to generate macrophages for antigen presentation experiments
We appreciated the low background activation and the consistent upregulation of activation markers only after defined stimulation. The cells maintained good viability through longer culture periods and performed well in flow staining, with clear population separation and minimal nonspecific staining artifacts. The format size was convenient for both pilot and main runs without switching lots.
Our team used these monocytes to generate macrophages for bacterial challenge assays
The cells showed excellent functional responsiveness, with consistent cytokine release and phagocytic activity after differentiation. We also valued the practical documentation and lot identification, which helps with internal QA and publication traceability.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.