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Human PB CD14+ Monocytes (Age: 61), 5 x 10^7 cells (MTS-1022-JF53)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Can I use these monocytes as a consistent starting point for macrophage differentiation and polarization studies?

Yes. CD14+ monocytes are a common precursor for macrophage differentiation, and having a cryopreserved, flow-characterized starting population helps standardize your baseline before polarization (e.g., classically activated vs alternative activation-like conditions). With 5×10^7 cells/vial, you can run differentiation plus phenotype/functional panels without constant resourcing interruptions.

How do you recommend we integrate this lot into a multi-timepoint experimental design?

For time-course designs, consistency is everything: thaw once, seed all time points in parallel when feasible, and keep media and stimulation timing tightly controlled. If your study spans weeks, securing sufficient vials from the same lot reduces variability. We can also advise on an allocation plan that preserves enough cells for QC at each stage.

What storage conditions are required immediately after delivery?

Upon receipt, transfer the vial directly from dry ice to liquid nitrogen storage (-150°C to -190°C). Temperature excursions can compromise viability; the handling notes emphasize that viability cannot be guaranteed if the recommended procedure is not followed.

The product labeling made it easy to track donor age and vial identity across experiments
These monocytes were integrated into a screening workflow where reproducibility is more important than chasing maximal yields from fresh donors. The batch performed consistently after thaw, and our marker panels were clean. Importantly, the behavior across replicate wells was predictable, which is essential for plate-based assays.
Overall, it's a reliable, scalable option for RUO immunology projects that need consistent monocyte starting material
Our group runs macrophage polarization studies and often struggles with variability introduced by mixed PBMC populations. This CD14+ monocyte prep improved our baseline stability and reduced background signals in our multiplex cytokine assays.
From a practical perspective, this product reduced a lot of friction
We didn't have to coordinate fresh donors, yet the cells performed well enough for serious functional work. Thawing and seeding were straightforward, and the culture remained stable with routine media changes. The consistency helped us hit milestones faster and reduced repeat runs.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.