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Rat Bone Marrow Monocytes (with Luciferase Expression), 0.5 x 10^6 cells (MTS-1022-JF98)

Datasheet

Overview

Description

With Luciferase expression, this product is isolated from the bone marrow of pathogen-free laboratory rat.
0.5 x 10^6 cells are delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Rat

Product Type

Immune cells

Specification

Source

Bone marrow

Format

Cryopreserved

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

0.5 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

How should I design controls to interpret luciferase signal correctly?

Include (1) non-luciferase monocytes or blank wells for background, (2) a fixed cell-number standard curve on each plate/day, and (3) condition-matched controls if your stimuli may affect metabolic state (which can indirectly affect luminescent output). This keeps luminescence tied to biology rather than plate-to-plate artifacts.

Will luciferase interfere with macrophage polarization or activation assays?

Luciferase is typically used as a reporter and does not inherently define polarization-but activation conditions can affect cellular metabolism and thus luminescence. The safest approach is to treat luminescence as one measurement layer and pair it with orthogonal macrophage markers (surface markers, cytokines) for mechanistic clarity.

What storage/handling factors most commonly reduce luciferase signal quality?

The top culprits are poor cold-chain handling and freeze-thaw stress. The page specifies storage transfer to liquid nitrogen and warns against repeated freezing and thawing-those steps help protect overall cell health and signal performance.

These luciferase monocytes integrated smoothly into our screening pipeline
We often evaluate multiple formulations and need rapid, plate-based metrics of survival and relative cell load. The luminescence signal was consistent and easy to normalize, and the cells tolerated typical manipulations without dramatic signal loss. The vendor's guidance on recovery and plating helped avoid unnecessary stress.
It saved us significant time compared with generating labeled cells in-house
Our laboratory needed luciferase-labeled primary monocytes to assess retention and persistence in a 3D matrix system. The signal allowed us to quantify cell presence over time without repeatedly disrupting the culture. The cells were robust enough for embedding and maintained reliable readouts during the assay window. We also ran stimulation experiments and confirmed that the cells remained responsive, which is critical for interpreting results.
It's a strong tool for high-throughput functional readouts
We used these luciferase monocytes to validate an immunotoxicity panel where we monitor cell survival and activation across multiple stimuli. The luminescence output provided a clean, quantitative baseline and responded in a way that correlated well with other viability markers.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.