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Mouse Bone Marrow Monocytes (with GFP Expression), 1 x 10^6 cells (MTS-1022-JF92)

Datasheet

Overview

Description

With GFP expression, this product is isolated from the bone marrow of pathogen-free laboratory C57BL/6 mouse.
1 x 10^6 cells are delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Mouse

Product Type

Immune cells

Specification

Source

Bone marrow

Format

Cryopreserved

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Will GFP signal remain stable after differentiation into macrophages?

In many workflows, reporter signal remains detectable through differentiation, but intensity can shift with activation state, culture duration, and instrument settings. We recommend a short pilot: thaw, recovery, differentiation, stimulus panel, then lock exposure/gating parameters. If you need higher-confidence tracking, we can suggest complementary approaches (e.g., dual readouts with surface markers).

What are your recommended receiving and storage steps to protect fluorescence performance?

Handle as a cryopreserved product: ship on dry ice, transfer to liquid nitrogen immediately on arrival, and avoid repeated freeze-thaw cycles. These steps help preserve both viability and functional performance.

Do you offer matched unlabeled controls for clean comparative design?

Yes. Many teams run GFP+ and wild-type monocytes in parallel to confirm the label doesn't influence the phenotype of interest. Share your study goal, and we'll recommend a matched control strategy that keeps interpretation tight and publication-ready.

We ordered this product for a tumor microenvironment model
The cells were robust and differentiated well, with GFP remaining detectable during the workflow. This improved our ability to quantify macrophage behavior in mixed cultures and helped generate cleaner datasets for analysis. The format size allowed us to run multiple conditions and controls without pooling from different sources.
Our team used the GFP+ monocytes for phagocytosis assays where we needed to distinguish macrophages from fluorescently labeled targets
The stable GFP signal made population identification unambiguous, and we saw consistent assay performance across replicates. The cells maintained good viability during extended imaging sessions and did not show abnormal activation behavior. This saved us from complex staining panels and reduced compensation issues in flow.
A very practical tool for imaging-heavy workflows
We used these GFP+ monocytes to validate a macrophage infiltration assay in a microfluidic device. The fluorescent signal made it easy to quantify movement and localization over time, and the cells maintained viability during imaging workflows. Differentiation into macrophages proceeded smoothly, and the GFP expression remained detectable throughout. This eliminated repeated dye labeling and reduced variability across runs.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.