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Human PB CD14+ Monocytes (Age: 33), Negative selected, 5 x 10^7 cells (MTS-1022-JF28)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood by removing other cell types using immunomagnetic negative selection, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Isolation Method

Negative selection

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Can I use these for monocyte-derived macrophage drug screening without confounding activation artifacts?

Yes, but pair the product choice with process discipline: endotoxin-controlled reagents, consistent recovery time, and standardized plating. Include baseline and vehicle controls on every plate, and consider adding an early time point to detect any handling-induced activation before drug exposure.

Does the negative-selection process reduce yield or robustness after thaw?

With proper thaw and recovery, robustness should remain strong. The largest determinant of yield is temperature stability and gentle processing. Avoid "extra washes" unless needed; each step can reduce recovery and increase stress.

What's the best way to build a donor-consistent macrophage bank from this vial?

Differentiate the full vial in a controlled run, then cryopreserve macrophages in assay-sized aliquots (if your protocol supports it) so downstream experiments stay donor-consistent. Validate phenotype after re-thaw before scaling this strategy.

We chose the negative-selected Age 33 5 × 10^7 monocytes to support RNA profiling after short stimulations
Compared with previous sources, baseline signatures were calmer and the induced response was easier to interpret. The large vial size let us run optimization and the main experiment without switching donors. Handling notes were clear and consistent with best practices, and packaging held temperature well.
Good choice for large co-culture builds
We generate macrophages for co-culture with tumor spheroids and needed a stable precursor. This vial gave us enough cells to build multiple plates while keeping donor consistency. Baseline activation was manageable, and stimulated responses were robust.
The biggest win for our team was planning
Cryopreserved format, clear LN2 storage instructions, and a vial size aligned with our weekly runs. The negative selection feature also reduced background drift across operators.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.