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Human PB CD14+ Monocytes, 1-5 x 10^7 cells (MTS-1022-JF61)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 x 10^7 cells, 1.5 x 10^7 cells, 2.5 x 10^7 cells, 4 x 10^7 cells, 5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What is the best downstream use case for these PB CD14+ monocytes, and why do you position them as an "ideal model"?

These PB CD14+ monocytes are widely used as precursors for macrophage and dendritic cell generation, making them highly practical for projects that require reproducible differentiation and phenotype modulation. Mechanistically, CD14 is a key myeloid marker involved in immune recognition, so CD14-enriched monocytes provide a clean starting point for macrophage biology studies such as polarization, cytokine profiling, and immune interaction assays.

I'm scaling an experiment-how should I choose between 1×10^7, 1.5×10^7, 2.5×10^7, 4×10^7, and 5×10^7 cells?

A practical way is to plan backward from your final plated cell density + differentiation yield expectations. Use smaller sizes for assay development and protocol optimization, and larger sizes when you need multi-arm studies (e.g., multiple stimuli, time-course sampling, or replicate-heavy omics). Since this listing offers multiple sizes, you can scale without changing the biological starting material category.

What are the most critical handling steps to protect viability after delivery?

These cells are shipped on dry ice and must be transferred immediately to liquid nitrogen (-150°C to -190°C) upon receipt. Avoid repeated freeze-thaw cycles, as they can compromise recovery, plating efficiency, and functional responses post-thaw.

Overall, the product quality and logistics reduced uncertainty in our schedule
We used the 1.5 × 10^7 CD14+ monocyte format for a screening campaign where cell availability and timing were critical. The cells thawed evenly, with minimal clumping, which is often our biggest pain point. We saw stable baseline behavior and strong dynamic range after activation, which improved our assay sensitivity.
Our lab runs macrophage polarization studies weekly, so we value suppliers who keep variability low
This CD14+ monocyte batch integrated smoothly into our pipeline; differentiation was efficient, and the cells showed expected morphology and adherence patterns. We also noted low debris and a clean population profile after initial gating. The support team was responsive when we asked about recommended handling and optional phenotyping markers.
A dependable option for labs that need reproducibility
The cells performed consistently across replicate plates and different media conditions, which gave us confidence that differences we observed were driven by our variables, not the cell input. The post-thaw workflow was uncomplicated, and viability looked solid without excessive resting or recovery steps.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.