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Human PB CD14+ Monocytes (Age: 49), 5 x 10^7 cells (MTS-1022-JF41)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

For a 5×10^7 cell lot, how should we plan aliquoting to support multi-arm studies without compromising quality?

The best approach is to aliquot once immediately after thaw (or, ideally, use multiple vials if provided) and avoid repeated temperature cycling. Allocate cells by study arm (e.g., baseline differentiation, stimulus panel, time course, omics) and prioritize sensitive workflows (RNA, phospho-signaling) early in the run. We can help you translate your protocol into an aliquot map that reduces waste and preserves consistency across arms.

Can these PB CD14+ monocytes be used for monocyte-derived dendritic cell (moDC) generation as well?

In many projects, yes. CD14+ PB monocytes are a common starting population for moDC differentiation with appropriate cytokine cocktails. The key is choosing the differentiation conditions and validation markers that match your application (antigen presentation, migration, maturation response).

How do you assess "functional readiness" beyond surface markers?

Surface markers are necessary but not sufficient. Functional readiness is best demonstrated with performance assays matched to your biology-phagocytosis capacity, cytokine secretion patterns after defined stimuli, metabolic shifts, or antigen presentation-related readouts. We can recommend a compact functional panel that provides high confidence without adding excessive time or cost.

This vial size let us run everything from a single donor source
Post-thaw recovery was strong, differentiation was uniform, and our stimulation assays produced consistent, interpretable shifts. The larger scale also improved scheduling: we could plan week-by-week without worrying about re-ordering or switching materials midstream.
Strong yield for polarization + rescue experiments
In polarization studies, we often need enough cells to repeat conditions, add rescue arms, and still keep consistent cell density. The 5 × 10^7 size gave us the flexibility to run multiple timepoints and confirm findings without stretching the biology. The cells responded well to polarization stimuli and maintained viability throughout the timeline.
Dependable documentation and quality assurance
We generated macrophages for bulk RNA-seq and targeted pathway analysis from this vial, and the results were consistent across replicates. Expression changes aligned with expected biology, and we didn't see unusual stress signatures that could confound interpretation. The ability to keep the entire study within one donor/lot reduced technical noise and strengthened the statistical story.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.