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Human M2 Macrophages (M-CSF), monocyte-derived (MTS-1223-HM5)

Datasheet

Overview

Description

Creative Biolabs offers non-activated, fully qualified M2- (M-CSF-) polarized cells which are produced from monocytes.

Species

Human

Product Type

Cell

Specification

Cell Source

Monocytes

Marker expression

CD163 positive; CD68 positive

Size

1,500,000 cells; 5,000,000 cells

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Are these M2 macrophages suitable for co-culture assays with epithelial or stromal cells?

Yes. They have been used successfully in co-culture systems with epithelial cells, fibroblasts, endothelial cells, and other stromal cell types. In such settings, they provide a physiologically relevant source of M2-like signals that can influence barrier function, extracellular matrix remodeling, and tissue homeostasis. Their stable M2 phenotype supports multi-day co-culture experiments with consistent behavior.

How do these cells respond to attempts at repolarization or phenotype switching?

One of the strengths of this product is the ability to study macrophage plasticity. Under appropriate stimulation with pro-inflammatory cytokines such as IFN-γ and TLR ligands, these M2 macrophages can partially shift toward a more inflammatory profile.

Are these cells compatible with transcriptomic and proteomic analyses?

Absolutely. These M2 macrophages have been successfully used in gene-expression profiling, proteomic studies, and secretome analysis. Their well-defined phenotype and high viability provide a solid foundation for high-resolution omics experiments. We recommend following best practices for RNA and protein extraction to preserve sample quality and ensure accurate downstream analysis.

These M-CSF-derived M2 macrophages have been extremely valuable for our tissue remodeling studies
They show a very clear M2 signature with strong CD163/CD206 expression and high IL-10 production. The cells perform consistently in co-culture systems with fibroblasts, which has helped us model pro-resolving environments more accurately.
From an omics perspective, these macrophages are excellent
We have generated high-quality RNA-seq and proteomics datasets from them, with clear M2-associated signatures that align well with the literature. The combination of consistency and viability makes them a reliable starting material.
Overall, we are very pleased with this product
The M2 phenotype is strong, the handling is straightforward, and the functional outputs are exactly what we needed for our regenerative immunology project. We plan to continue using these cells in future studies.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.