Macrophage Chemokine Ligand 4 (CCL4) Competition ELISA Kit (MTS-1123-HM18)

The competitive kit is designed with a shorter overall assay time (listed at about 1.5 hours) and is often chosen when labs need faster turnaround. Faster does not automatically mean lower quality-what matters most is disciplined execution: accurate pipetting, consistent timing, and proper mixing. Competitive formats can be very robust when run correctly, but the curve is typically inverse and can be more sensitive to small handling differences. If your priority is speed plus consistency, we recommend running a pilot to confirm curve stability and including a fixed internal control sample on every plate to track performance.

Yes, tissue homogenate is listed among compatible sample types, and many users successfully quantify chemokines in homogenates with competitive formats. The practical success factors are (1) strong clarification (centrifugation to remove particulates), (2) selecting a dilution that reduces matrix interference, and (3) confirming that dilution produces a linear response. If background is high, it often reflects residual debris, lipid content, or insufficient dilution rather than the assay format itself. We recommend spike-recovery checks during setup so you can confirm that the matrix is not suppressing or inflating the apparent CCL4 concentration.

Using a single dilution factor across very different matrices can be risky because each matrix has different protein composition and potential interferents. Instead, treat each sample type as its own optimized condition: determine an appropriate dilution for supernatants, and separately determine an appropriate dilution for serum/plasma. Then maintain that dilution consistently within each cohort and include matrix-matched controls. You can still compare biological trends across matrices, but do so cautiously, and avoid assuming that identical dilution implies identical matrix behavior. A short pilot with dilution linearity testing is usually the fastest path to defensible comparisons.