Macrophage Chemokine Ligand 22 (CCL22) ELISA Kit, Colorimetric (MTS-1123-HM22)

This colorimetric sandwich ELISA is designed for quantitative measurement of CCL22 and is commonly applied to serum, plasma, cell culture supernatant, and tissue homogenate. Running different matrices on the same plate is possible, but you should treat them as separate "assay groups" with their own dilution plans and matrix blanks. Tissue homogenates often require clarification steps and may need higher dilution to reduce interference. For best comparability, keep each matrix within the linear range, verify dilution parallelism, and use consistent handling so matrix effects do not distort the calculated concentrations.

Yes, it's realistic. A standard sandwich ELISA workflow typically fits within a working day, including plate incubations, washes, and color development. The key is organization: prepare standards and sample dilutions in advance, ensure wash buffers are ready, and use a timer for each step. To maintain consistency, avoid extending incubations unevenly across plates and read the plate promptly once color development reaches the recommended range. If you anticipate interruptions, it's better to pause at a validated step (e.g., after washes) according to the protocol rather than improvising mid-reaction.

ELISA performance depends on preserving antibody activity and preventing microbial contamination. Store components at the recommended temperatures, keep caps tightly closed, and avoid repeated freeze-thaw cycles for standards or sensitive reagents by aliquoting when practical. Bring reagents to room temperature before use and mix gently without creating foam. Also, use clean pipette tips and reservoirs to prevent contamination. If you notice drifting standard curves, it often reflects inconsistent incubation temperature, washing variability, or reagent degradation from improper storage-so a simple storage and handling audit can restore performance quickly.