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Granulocyte-Macrophage Colony-Stimulating Factor 2 (CSF2) ELISA Kit, qPCR (MTS-1123-HM56)

Datasheet

Overview

Description

Creative Biolabs provides competition ELISA kit for quantitative measurement of Granulocyte-Macrophage Colony-Stimulating Factor 2 (CSF2) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Competition ELISA

Analytical Method

Quantitative

Sample Type

Cell Culture Supernatant, Plasma, Serum

Specificity

Granulocyte-Macrophage Colony-Stimulating Factor 2 (CSF2)

Specification

Size

96 tests

Sample Volume

25 µL

Plate

Pre-coated

Bioassay Target Name

Granulocyte-Macrophage Colony-Stimulating Factor 2 (CSF2)

Storage

4 °C, -20 °C, -80 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Synonyms

CSF; GMCSF

Background

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. This gene plays a role in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been detected in SARS-CoV-2 infected patients that develop acute respiratory distress syndrome. Mice deficient in this gene or its receptor develop pulmonary alveolar proteinosis.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM824	Human	0.2 pg/mL		Inquiry
MTS-1123-HM825	Mouse	0.6 pg/mL		Inquiry
MTS-1123-HM826	Pig	7.2 pg/mL		Inquiry
MTS-1123-HM827	Rat	1 pg/mL		Inquiry
MTS-1123-HM828	Rhesus Monkey	User optimized		Inquiry
MTS-1123-HM829	Cat	User optimized		Inquiry

FAQs Customer Reviews Related Products

The description mentions qPCR-can you explain how this fits with protein quantification needs?

This page positions the kit as an ELISA solution with "qPCR" labeling, which many teams interpret as supporting a combined strategy: protein-level measurement by ELISA plus transcript-level confirmation by qPCR. In practice, ELISA captures secreted CSF2 protein changes, while qPCR can help confirm upstream expression trends. We can advise on aligning sampling, normalization controls, and how to report concordance clearly.

If ELISA and qPCR trends disagree, what should I check first?

Discordance can be biological (transcription vs secretion timing) or technical (sample handling, inhibitors, normalization). First, confirm matched timepoints and sample identity, then check qPCR housekeeping stability and ELISA matrix effects. Also consider that cytokine protein can persist longer than mRNA, or secretion may lag. We recommend a short time-course pilot to map kinetics and select the most informative collection window.

Is this approach acceptable for translational studies where reviewers expect orthogonal validation?

Yes. Many reviewers appreciate orthogonal evidence, especially for cytokines with complex regulation. We recommend documenting assay controls, showing repeatability, and clearly stating whether the ELISA result is quantitative or used for relative comparisons. Pairing ELISA with qPCR can strengthen mechanistic claims if you present kinetics and controls transparently. We can suggest a reporting template for methods and QC.

Worked well as part of our ELISA-plus-qPCR cytokine validation pipeline
We tracked CSF2 protein secretion by ELISA and compared it with expression changes measured by qPCR. The kit was easy to integrate, and protein trends matched our expected stimulation responses at later timepoints. When early timepoints diverged, it helped us realize secretion lagged behind transcription. Overall, it supported a clearer story and improved confidence in our conclusions.
Practical workflow for teams that want protein readout with transcript context
Our group routinely pairs cytokine protein measurement with gene expression to satisfy internal QC. The ELISA workflow was familiar and the data were consistent after we standardized a pooled QC sample. The combined view helped us interpret complex responses where mRNA spikes did not immediately translate to high protein levels. Useful for building mechanistic narratives in reports.
Good for trend confirmation; helped resolve ambiguous cytokine release findings
We had borderline CSF2 changes in supernatants and wanted stronger evidence before committing to downstream experiments. Running ELISA results alongside qPCR trends helped us decide which conditions were real responders. The kit's protocol was manageable, and guidance on timing and controls helped us avoid drawing conclusions from mismatched collection windows.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.