Macrophage Mannose Receptor 1 (MRC1) ELISA Kit, qPCR (MTS-1123-HM2)

This product is presented as a sandwich ELISA workflow with a qPCR-based readout and is described as semi-quantitative on the page. Practically, you should interpret results as relative or comparative signals rather than absolute concentrations unless your experimental design includes appropriate calibrators and controls. We recommend running consistent reference samples across runs and focusing on fold-changes between conditions, especially when comparing treatment groups or time courses.

If your primary need is robust absolute quantification using standard plate readers, the colorimetric ELISA is typically the simplest route. The qPCR readout can be attractive when sample volume is limited or when you prefer a nucleic-acid amplification-style detection step in your lab workflow. Because this format is labeled semi-quantitative, it's best suited for comparing groups under matched conditions rather than building cross-study concentration benchmarks.

Matrix inhibitors can affect downstream amplification-like steps, so controls are essential. We suggest including a no-sample control, a matrix blank (your sample buffer without analyte), and spike-in recovery in representative sample types. If you see suppression, dilute the sample and confirm that the observed signal changes proportionally (parallelism). Keeping sample processing consistent and avoiding carryover of harsh lysis reagents will help maintain reliable semi-quantitative comparisons.