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Macrophage Cannabinoid Receptor 2 (CNR2) ELISA Kit, Colorimetric (MTS-1123-HM5)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for quantitative measurement of Cannabinoid Receptor 2 (CNR2) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Analytical Method

Quantitative

Sample Type

Plasma, Serum, Tissue Homogenate

Specificity

Cannabinoid Receptor 2 (CNR2)

Specification

Size

96 tests

Sample Volume

100 μL

Assay Time

3 h

Plate

Pre-coated

Bioassay Target Name

Cannabinoid Receptor 2 (CNR2)

Storage

4 °C, -20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

cannabinoid receptor 2

Synonyms

CB2; CX5; CB-2

Background

The cannabinoid delta-9-tetrahydrocannabinol is the principal psychoactive ingredient of marijuana. The proteins encoded by this gene and the cannabinoid receptor 1 (brain) (CNR1) gene have the characteristics of a guanine nucleotide-binding protein (G-protein)-coupled receptor for cannabinoids. They inhibit adenylate cyclase activity in a dose-dependent, stereoselective, and pertussis toxin-sensitive manner. These proteins have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. The cannabinoid receptors are members of family 1 of the G-protein-coupled receptors.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM134	Human		0.15 ng/mL-10 ng/mL	Inquiry
MTS-1123-HM135	Rat		0.15 ng/mL-10 ng/mL	Inquiry
MTS-1123-HM136	Mouse		62.5 pg/mL-4000 pg/mL	Inquiry
MTS-1123-HM137	Human		62.5 pg/mL-4000 pg/mL	Inquiry

FAQs Customer Reviews Related Products

CNR2 is a GPCR membrane protein. Is ELISA the right approach, and what sample type usually gives the cleanest signal?

GPCRs can be challenging, but ELISA can work well when sample preparation is optimized. In most cases, clarified cell lysates with compatible detergent levels provide cleaner, more consistent signal than crude membrane preparations. Because the kit is intended for quantitative measurement in different sample types, we recommend starting with a mild lysis approach, confirming linearity with serial dilution, and using spike recovery to verify that the matrix does not suppress binding or increase background.

How can I ensure I'm measuring CNR2 protein changes rather than just differences in cell number or viability across treatments?

Normalize thoughtfully: measure total protein concentration for each lysate, load equal protein per well, and consider pairing with a viability or cell-count readout. Including a housekeeping protein assay (or a consistent reference lysate) helps distinguish true receptor regulation from sampling variation. If treatments affect adherence or survival, normalize to both total protein and cell number where possible; agreement between these normalizations increases confidence in biological interpretation.

I'm running samples with high serum content. Could serum components interfere, and what's a practical way to check?

Serum can add background and introduce binding competitors. A practical check is a spike-and-recovery experiment: add a known amount of target (or a positive control sample) into your serum-containing matrix and compare measured versus expected values. If recovery is poor, dilute the matrix, switch to serum-free collection before harvest, or perform a cleanup/clarification step. Always run matrix blanks alongside standards to detect non-specific color development.

Effective CNR2 ELISA after optimizing mild lysis and normalization
We used this kit to quantify CNR2 changes following macrophage stimulation. As expected for a membrane receptor, sample prep mattered more than the plate steps: once we reduced detergent and normalized by total protein, the data stabilized and matched our qPCR trend. The colorimetric readout is convenient and doesn't require specialized equipment. If you plan ahead with dilution linearity and spike recovery, it's a very workable approach.
Good screening assay for treatment comparisons with proper controls
We screened several compounds for effects on CNR2 expression and the kit helped rank-order responses across conditions. The biggest improvement came from adding a pooled reference lysate on every plate and sealing plates during incubation to avoid edge effects. Results were reproducible across days when we kept lysis and clarification consistent. It's not a substitute for functional receptor assays, but it's useful for protein-level screening.
Straightforward protocol and responsive support for membrane-protein questions
The ELISA workflow was standard, but we had questions about handling a GPCR target. Support guidance on dilution series and buffer compatibility was helpful. After implementing those suggestions, background decreased and replicate variability improved. The kit performed reliably for relative comparisons between macrophage states. For anyone measuring CNR2, plan time for sample-prep optimization-once done, the assay is easy to scale.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.