Granulocyte-Macrophage Colony-Stimulating Factor 2 (CSF2) Antibody ELISA Kit, Colorimetric (MTS-1123-HM57)

This page describes a colorimetric sandwich ELISA kit for CSF2 measurement, labeled as an "Antibody ELISA Kit." Operationally, you should treat it as an ELISA-based detection workflow where antibodies are the capture/detection reagents enabling CSF2 quantification. If your goal is to evaluate anti-CSF2 antibodies (e.g., ADA), please tell us-those assays require different formats and controls than cytokine quantification.

Yes, that's a common application. We recommend collecting supernatants at consistent timepoints, clarifying samples to remove debris, and running technical replicates. Include a common pooled QC sample across plates to compare M1/M2 conditions reliably over multiple runs. If you're mapping kinetics, a short time-course pilot can help choose a window where CSF2 differences are most separable.

Heterophilic antibodies and rheumatoid factor can cause non-specific bridging in immunoassays. To mitigate, dilute samples, use appropriate blocking strategies, and include matrix-matched controls where feasible. If you suspect interference, compare results with and without heterophilic blocking reagents and look for non-linear dilution behavior. We can help design an interference check so your reported CSF2 signal is trustworthy.