Erythroblast Macrophage Protein (MAEA) ELISA Kit, Colorimetric (MTS-1123-HM13)

This MAEA kit is positioned for cell culture cell-based measurement rather than typical serum/plasma or tissue homogenate workflows. In practice, you'll get the most reliable data when you follow the recommended cell handling steps in the protocol and keep conditions consistent across wells (cell number, culture time, and treatment exposure). If your study goal is secreted MAEA in supernatants or MAEA in complex tissue matrices, that is a different validation scope-please share your sample type and species so we can recommend the most appropriate assay format or optimization approach for your project.

An uncoated plate usually provides flexibility, but it also means consistency becomes more dependent on how strictly you follow the protocol and standardize your workflow. To keep reproducibility high, we recommend using the same plate type across experiments, preparing reagents fresh as instructed, and controlling incubation time/temperature as tightly as possible. For cell-based readouts, it's also important to keep edge effects in mind (e.g., avoid uneven evaporation and maintain uniform seeding). If you tell us your cell line and plate handling preferences, we can share practical tips to reduce variability and improve plate-to-plate agreement.

For multi-species comparisons, the most important step is selecting the kit/reactivity option that matches each species you will test, because antibody recognition can differ significantly even when proteins are homologous. This MAEA kit line includes species options such as Human/Mouse/Rat and Chicken with user-optimized performance depending on sample context. We strongly suggest running a small pilot first (a few representative samples per species) to confirm signal behavior, then locking your dilution and handling conditions before scaling up. If you share the species list and sample format (cells only vs. mixed matrices), we can help you plan that pilot efficiently.