Natural Resistance-Associated Macrophage Protein 2 (NRAMP2) ELISA Kit (MTS-1123-HM123)

This NRAMP2 ELISA kit is listed with multiple reactivity options, including Rat, Human, and Mouse variants. First, match the kit to your target species to ensure antibody compatibility. For uncommon matrices (tissue lysates, iron-rich samples, or detergent-containing buffers), we recommend a pilot optimization: test a small set of buffer compositions and run serial dilutions to assess linearity and recovery. If your lysate contains strong detergents, consider diluting further or using a milder lysis buffer to reduce assay interference. Including spike-in recovery controls is a practical way to confirm that your matrix is not suppressing the signal.

In oxidative or inflammatory models, sample composition can change dramatically (protease activity, hemolysis risk, and altered total protein), which may introduce variability unrelated to true NRAMP2 abundance. Practical steps include adding protease inhibitors for lysates, clarifying samples by centrifugation, normalizing input by total protein for lysates, and avoiding repeated freeze-thaw cycles. If comparing conditions that differ in cell death, include viability/cell count normalization or a housekeeping protein to interpret whether changes reflect expression shifts versus cellular composition. Running matched controls and spike-recovery checks can help confirm that the observed differences reflect biology, not matrix-driven assay artifacts.

"User optimized" indicates that sensitivity and detection range are intended to be optimized by the user depending on sample type, preparation, and expected abundance, rather than a single universal performance window being fixed for all matrices. In planning, that means you should allocate a short pre-study to establish the working dilution range, confirm standard curve behavior in your hands, and estimate intra-/inter-assay precision using replicates. For interpretation, focus on relative differences within a controlled experiment (matched handling and timing) until you confirm absolute quantitation suitability for your matrix. Once optimized, document dilution factors and acceptance criteria so later runs remain comparable.