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Macrophage Interleukin 2 (IL2) ELISA Kit, Colorimetric (MTS-1123-HM67)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for quantitative measurement of Interleukin 2 (IL2) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Analytical Method

Quantitative

Sample Type

Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Specificity

Interleukin 2 (IL2)

Specification

Size

96 tests

Sample Volume

100 μL

Assay Time

3 h

Plate

Pre-coated

Bioassay Target Name

Interleukin 2 (IL2)

Storage

4 °C, -20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

interleukin 2

Synonyms

IL-2; TCGF; lymphokine

Background

This gene is a member of the interleukin 2 (IL2) cytokine subfamily which includes IL4, IL7, IL9, IL15, IL21, erythropoietin, and thrombopoietin. The protein encoded by this gene is a secreted cytokine produced by activated CD4+ and CD8+ T lymphocytes, that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2R) is a heterotrimeric protein complex whose gamma chain is also shared by IL4 and IL7. The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM491	Human		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM492	Human		1.56 pg/mL-100 pg/mL	Inquiry
MTS-1123-HM493	Mouse		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM494	Rat		31.2-2000 pg/mL	Inquiry
MTS-1123-HM495	Rat		6.25 pg/mL-400 pg/mL	Inquiry
MTS-1123-HM496	Rabbit		1.56 pg/mL-100 pg/mL	Inquiry
MTS-1123-HM497	Chicken		1.56 pg/mL-100 pg/mL	Inquiry
MTS-1123-HM498	Dog		1.56 pg/mL-100 pg/mL	Inquiry
MTS-1123-HM499	Pig		1.56 pg/mL-100 pg/mL	Inquiry
MTS-1123-HM500	Cow		1.56 pg/mL-100 pg/mL	Inquiry
MTS-1123-HM501	Sheep		31.25 pg/mL-2000 pg/mL	Inquiry
MTS-1123-HM502	Hamster		12.5 pg/mL-800 pg/mL	Inquiry
MTS-1123-HM503	Goat		31.25 pg/mL-2000 pg/mL	Inquiry
MTS-1123-HM504	Duck		6.25 pg/mL-400 pg/mL	Inquiry
MTS-1123-HM505	Horse		31.25 pg/mL-2000 pg/mL	Inquiry
MTS-1123-HM506	Guinea Pig		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM507	Monkey		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM508	Cat		25-6000 pg/mL	Inquiry
MTS-1123-HM509	Rhesus Monkey		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM510	Dolphin		User optimized	Inquiry
MTS-1123-HM511	Guinea Pig		User optimized	Inquiry

FAQs Customer Reviews Related Products

IL-2 is often low in macrophage-only cultures-does this kit still make sense for my study?

It depends on your biology. IL-2 is classically associated with T cells, but macrophage-related projects may measure IL-2 in co-culture systems or immune-stimulation contexts. If your system is macrophage-only, we suggest a small pilot test with stimulated conditions and positive controls to confirm that IL-2 is present at measurable levels. If signals remain near background, pairing with a higher-producing model or co-culture may be more informative.

Can I use this kit for co-culture supernatants where both T cells and macrophages are present?

Yes, co-culture supernatants are a common use case for IL-2 measurement, especially when evaluating macrophage-driven modulation of T cell activation. To ensure reliable results, clarify the supernatant, avoid repeated freeze-thaw, and consider running a dilution series because co-cultures can produce a wide dynamic range. Including monoculture controls helps you interpret whether macrophages are amplifying or suppressing IL-2 output.

How do I prevent plate-to-plate drift when I run IL-2 across multiple weeks?

Plate drift is usually controlled by consistent execution and good anchors. Run the standard curve on every plate, include the same internal reference sample (aliquoted and frozen once), and keep incubation times identical across runs. Also standardize wash method and reader settings. This "process discipline" often matters more than any single reagent choice when you want IL-2 data that remain comparable over time.

Worked well for T cell-macrophage co-culture IL-2 monitoring
We used the kit for co-culture experiments and saw IL-2 shifts that tracked with our activation markers. The assay was easy to integrate into our routine and didn't add complexity. We found that running at least one dilution per sample prevented saturation in high-producing conditions. Overall, it gave consistent IL-2 trends that supported our conclusions about immune modulation in the co-culture system.
Good for screening immunomodulatory compounds in a mixed-cell setup
We screened compounds expected to alter IL-2 output and the kit provided clear differentiation between active and inactive conditions. The biggest benefit was speed: we could run many conditions in parallel and make decisions quickly. With consistent sample handling, replicate variability was low. It's a good choice if you're focused on trend detection and condition ranking rather than ultra-deep analytical characterization.
Reliable results after we added strict controls and an internal reference sample
Our first attempt had some plate-to-plate variation. After adding a frozen internal reference aliquot and keeping incubation timing consistent, the data became much more stable. We also included monoculture controls, which made interpretation cleaner. With these basic best practices, the kit performed reliably and helped connect IL-2 output to our functional readouts in immune activation assays.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.