Macrophage Scavenger Receptor 1 (MSR1) ELISA Kit, Colorimetric (MTS-1123-HM3)

Yes-membrane-associated targets are commonly measured from lysates, but extraction efficiency is critical. Use a gentle lysis method that solubilizes membrane proteins without excessive denaturation, clarify the lysate to remove debris, and run a dilution series to verify linearity. Because this is a sandwich ELISA intended for quantitative measurement across different sample types, optimizing lysis conditions and verifying recovery in your matrix will produce the most dependable results.

Lipid-rich matrices can increase background in colorimetric ELISA. We recommend additional clarification steps (higher-speed centrifugation) and, if compatible with your biology, modest sample dilution to reduce interfering components. Ensure thorough washing and avoid over-incubation with detection reagents. Including matrix blanks and spike recovery using representative samples is the fastest way to confirm that the observed signal is MSR1-specific rather than matrix-driven.

You can, but comparisons are strongest when sample preparation is matched as closely as possible. Cell lines and primary cells often differ in protein content and matrix complexity, which can shift apparent recovery. We recommend quantifying total protein and normalizing input, then confirming parallelism for each sample type. If matrices differ substantially, consider separate dilution strategies and include a shared internal control sample to anchor plate-to-plate and model-to-model comparisons.