Macrophage Chemokine Ligand 9 (CCL9) ELISA Kit, Colorimetric (MTS-1123-HM31)

The product overview describes it as a sandwich ELISA with a colorimetric detection method and emphasizes quantitative measurement of CCL9. For best quantitative performance in your lab, we recommend building a full standard curve on every plate, running samples in duplicate, and confirming that your chosen dilution falls within the linear portion of the curve-especially if your samples are complex matrices like serum or tissue-derived extracts.

Plate-to-plate reproducibility is usually improved by keeping the same operator timing, using calibrated pipettes, and including a consistent internal control sample. If you run multiple plates over weeks, freeze aliquots of a pooled "reference" sample and include it each run. That reference helps normalize day-to-day shifts (e.g., incubation timing or temperature differences). Since the kit is described for quantitative use, these controls are the simplest way to keep your dataset publication-ready.

Yes-conditioned media can be an excellent sample type for CCL9 detection, but strong stimulation can push concentrations above the top of the standard curve. We recommend planning dilution upfront: test at least two dilutions in the first run, then select the dilution that places your signals comfortably within the curve. This avoids re-running plates and improves your confidence in quantitative claims.