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Macrophage Elastase (MMP12) Competition ELISA Kit (MTS-1123-HM47)

Datasheet

Overview

Description

Creative Biolabs provides competition ELISA kit for quantitative measurement of Macrophage Elastase (MMP12) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Reactivity

Human

Detection Method

Colorimetric

Method Type

Competition ELISA

Analytical Method

Quantitative

Sensitivity

1.0 pg/mL

Sample Type

Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Specificity

Macrophage Elastase (MMP12)

Specification

Size

96 tests

Detection Range

100-2500 pg/mL

Sample Volume

100 μL

Assay Time

1.5 h

Plate

Pre-coated

Bioassay Target Name

Macrophage Elastase (MMP12)

Storage

4 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

matrix metallopeptidase 12

Synonyms

ME; HME; MME; MMP-12

Background

This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease degrades soluble and insoluble elastin. This gene may play a role in aneurysm formation and mutations in this gene are associated with lung function and chronic obstructive pulmonary disease (COPD). This gene is part of a cluster of MMP genes on chromosome 11.

FAQs Customer Reviews Related Products

Why would I choose a competition ELISA instead of sandwich ELISA for MMP12? What's the practical difference in daily lab work?

This product uses a competition ELISA format for quantitative measurement of MMP12 with a colorimetric readout. Competition ELISA can be advantageous when the target has limited accessible epitopes, or when sample conditions make two-antibody sandwich pairing less optimal. In practice, you interpret the signal inversely (higher analyte can mean lower signal), so plate layout and careful control handling are important. We provide guidance to ensure correct curve interpretation and consistent execution, especially for teams new to competition assays.

Our team is concerned about "inverse curves" and interpretation mistakes. How do you help prevent reporting errors?

We recommend running standards and key controls in duplicate or triplicate, and we encourage labs to keep a template plate map that clearly labels standards, blanks, and samples. Because competition assays behave differently than sandwich ELISA, we emphasize curve verification (a consistent monotonic trend) before accepting results. If you share your planned throughput and sample count, we can suggest a plate strategy to reduce mix-ups. We also help troubleshoot curve shape issues caused by timing variation, insufficient mixing, or inconsistent incubation.

Can this competition kit tolerate complex matrices (tissue homogenates, serum), and what validation steps are most important?

Complex matrices can be compatible, but we recommend validation steps that confirm the matrix is not distorting competition dynamics. Start with dilution linearity to see whether the apparent concentration scales predictably, then perform spike-and-recovery to confirm measurement integrity. If the matrix strongly interferes, we may suggest additional dilution or sample cleanup. Our technical support can guide these steps so you get interpretable quantitative data without multiple failed plates.

Competition format worked well when sandwich detection was inconsistent in our inflammation assay pipeline
We initially tried sandwich assays for MMP12 but struggled with inconsistent detection in our samples. This competition ELISA gave us more stable curves and allowed quantitative comparisons across conditions. The inverse-signal interpretation required careful attention, but the kit documentation and vendor tips helped prevent mistakes. After two runs, the workflow became routine and the results were reproducible. It's a good option if sandwich pairing is problematic in your matrix.
Clear quantitative trends once we standardized timing and mixing for routine lab and screening use
Our first attempt had a slightly odd curve, and support suggested we tighten timing consistency and improve mixing uniformity across wells. That advice solved the issue and we obtained clean, interpretable competition curves. We used the kit for tissue homogenates and serum comparisons and the quantitative trends matched our biological expectations. Overall, it's reliable, but like all competition ELISAs, it rewards disciplined technique and clear plate organization.
Good support team for training new users on competition ELISA
We had junior staff who were unfamiliar with inverse-signal ELISA formats. The supplier's guidance on plate mapping, control placement, and curve acceptance criteria reduced confusion and prevented data reporting errors. Once the team learned the interpretation, the assay performed consistently and helped us compare multiple experimental groups efficiently. If you need a competition format for MMP12, this kit is practical and the support reduces the learning curve.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.