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Macrophage Elastase (MMP12) ELISA Kit, Colorimetric (MTS-1123-HM46)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for quantitative measurement of Macrophage Elastase (MMP12) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Analytical Method

Quantitative

Sample Type

Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Specificity

Macrophage Elastase (MMP12)

Specification

Size

96 tests

Sample Volume

100 μL

Assay Time

3 h

Plate

Pre-coated

Bioassay Target Name

Macrophage Elastase (MMP12)

Storage

4 °C, -20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

matrix metallopeptidase 12

Synonyms

ME; HME; MME; MMP-12

Background

This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease degrades soluble and insoluble elastin. This gene may play a role in aneurysm formation and mutations in this gene are associated with lung function and chronic obstructive pulmonary disease (COPD). This gene is part of a cluster of MMP genes on chromosome 11.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM336	Rat		78.12 pg/mL-5000 pg/mL	Inquiry
MTS-1123-HM337	Human		0.31 ng/mL-20 ng/mL	Inquiry
MTS-1123-HM338	Mouse		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM339	Mouse		7.8 pg/mL-500 pg/mL	Inquiry
MTS-1123-HM340	Human		62.5-4000 pg/mL	Inquiry
MTS-1123-HM341	Mouse		62.5-4000 pg/mL	Inquiry
MTS-1123-HM342	Monkey		User optimized	Inquiry
MTS-1123-HM343	Cow		User optimized	Inquiry
MTS-1123-HM344	Chicken		User optimized	Inquiry
MTS-1123-HM345	Goat		User optimized	Inquiry
MTS-1123-HM346	Rabbit		User optimized	Inquiry
MTS-1123-HM347	Sheep		User optimized	Inquiry
MTS-1123-HM348	Guinea Pig		User optimized	Inquiry
MTS-1123-HM349	Sheep		User optimized	Inquiry
MTS-1123-HM350	Dog		User optimized	Inquiry

FAQs Customer Reviews Related Products

MMP12 can be present as pro-enzyme and active forms. Will this kit detect total MMP12, and how should I interpret the value biologically?

This is a sandwich ELISA intended for quantitative measurement of Macrophage Elastase (MMP12) using a colorimetric readout. Whether it reflects total versus form-specific detection depends on the antibody epitope recognition. Many users interpret ELISA as protein abundance rather than enzymatic activity. For activity-specific conclusions, we recommend pairing this kit with an activity assay or zymography. We can help you plan validation based on your sample type and hypothesis so your interpretation stays accurate.

We measure MMP12 in lung tissue homogenates, which can be messy. What sample prep tips reduce background and improve recovery?

For tissue homogenates, consistent processing matters: keep samples cold, clarify thoroughly to remove debris, and avoid harsh detergents that can increase non-specific signals. Since the kit is designed for quantitative measurement across different sample types, we recommend a pilot dilution series to identify the best dilution window for your matrix. Adding a spike-and-recovery check can confirm whether the matrix suppresses signal. If you share your homogenization buffer, we'll suggest practical improvements.

What level of technical support do you offer if we need help optimizing for a non-standard matrix or species model?

We support optimization planning for matrices that commonly challenge ELISA, including tissue homogenates and inhibitor-rich lysates. We can recommend dilution strategies, control selection, and validation checks like parallelism and recovery. If you use an uncommon species or engineered model, we'll help you assess expected performance and determine whether a pilot run is appropriate. Our goal is to reduce rework and help you reach interpretable data faster.

Clean quantitative performance in tissue homogenates after dilution optimization for complex matrices and tight schedules
We used this MMP12 kit on lung tissue homogenates where background can be high. After clarifying samples well and using a dilution series, the signal-to-noise improved significantly and the standard curve behaved well. The kit was useful for comparing treatment groups, and the quantitative readout aligned with our expectations from histology trends. Support provided helpful advice on recovery testing, which improved our confidence in the measurements.
Straightforward sandwich ELISA workflow for routine macrophage studies for consistent, publication-ready reporting
The assay ran like a standard sandwich ELISA and fit smoothly into our lab's weekly schedule. We measured MMP12 in macrophage-conditioned media and saw clear increases with pro-inflammatory stimulation conditions. The colorimetric readout was stable on our plate reader, and we appreciated the vendor's guidance that abundance does not always equal enzymatic activity. That helped us decide to add an activity assay for confirmation.
Good kit for screening before investing in deeper mechanistic assays
We needed a quick and reliable readout to screen multiple conditions and decide where to focus mechanistic experiments. This kit provided consistent quantitative trends across replicates and helped us prioritize two conditions for follow-up work. The workflow is approachable for any ELISA-capable team, and the vendor answered questions about matrix effects without delay. Overall, it's a strong screening tool when you need protein-level MMP12 comparisons.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.