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Macrophage IL-1 beta Competition ELISA Kit (MTS-1123-HM65)

Datasheet

Overview

Description

Creative Biolabs provides competition ELISA kit for quantitative measurement of IL-1 beta (IL1B) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Competition ELISA

Analytical Method

Quantitative

Sample Type

Plasma, Serum, Tissue Homogenate

Specificity

IL-1 beta (IL1B)

Specification

Size

96 tests

Sample Volume

50 μL

Assay Time

1 - 4.5 h

Plate

Pre-coated

Bioassay Target Name

IL-1 beta (IL1B)

Storage

4 °C, -20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

interleukin 1 beta

Synonyms

IL-1; IL1F2; IL1beta; IL1-BETA

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Similarly, IL-1B has been implicated in human osteoarthritis pathogenesis. Patients with severe Coronavirus Disease 2019 (COVID-19) present elevated levels of pro-inflammatory cytokines such as IL-1B in bronchial alveolar lavage fluid samples. The lung damage induced by the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL-1B. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM481	Chicken		0.27 pg/mL-200 pg/mL	Inquiry
MTS-1123-HM482	Cow		62.5-4000 pg/mL	Inquiry
MTS-1123-HM483	Rabbit		50-1000 pg/mL	Inquiry
MTS-1123-HM484	Mouse		50-1000 pg/mL	Inquiry
MTS-1123-HM485	Human		50-1000 pg/mL	Inquiry
MTS-1123-HM486	Cow		User optimized	Inquiry
MTS-1123-HM487	Dog		50-1000 pg/mL	Inquiry
MTS-1123-HM488	Chicken		User optimized	Inquiry
MTS-1123-HM489	Pig		50-1000 pg/mL	Inquiry
MTS-1123-HM490	Guinea Pig		50-1000 pg/mL	Inquiry

FAQs Customer Reviews Related Products

What's the practical advantage of a competition IL-1β ELISA for macrophage samples?

Competition ELISA can be helpful when you want a robust quantitative workflow across multiple sample types. This kit is listed as competition ELISA, colorimetric detection, and quantitative analysis, supporting plasma, serum, and tissue homogenate matrices. Many users find competition format useful when sample composition varies, as long as you confirm dilution linearity during initial setup.

How much sample do I need, and can I finish it in one afternoon?

The kit specifies 50 µL sample volume and an assay time of ~1 to 4.5 hours, with a pre-coated plate. That makes it feasible to complete within an afternoon depending on your incubation choices and plate-wash workflow. If you're running many samples, preparing standards and dilutions in advance is the easiest way to stay within the shorter end of the time window.

Can I store unused components and run another plate next month?

The listed storage conditions are 4°C and -20°C, and the expiry is 6 months. To preserve performance, reseal components tightly, avoid contamination during pipetting, and minimize freeze-thaw for any reconstituted reagents. We also recommend documenting your incubation times and reader settings so second-run data remain comparable to your first run.

Excellent IL-1β quantification across serum and tissue homogenate samples
We needed one assay approach that could handle both serum and tissue homogenates without turning into a troubleshooting project. After a quick pilot dilution, the competition format produced stable curves and reproducible values. The time window was realistic, and we liked the modest 50 µL sample requirement. Overall, it delivered dependable IL-1β numbers that matched our inflammatory model expectations.
Pre-coated plate and clear workflow made it easy for shared lab use
In a shared lab, consistency matters more than fancy features. The pre-coated plate reduced variability and speeded up setup. We appreciated that the kit clearly states sample types and practical assay timing, which helps plan runs. Replicates were tight when we followed washing carefully. It's a solid choice if multiple people will run the same IL-1β assay.
Good sensitivity and clean data once we standardized sample handling
Our first run had slightly higher background due to inconsistent homogenate clarification. After standardizing centrifugation and dilution, the assay produced clean, interpretable data. The 96-test size was perfect for running controls, duplicates, and a couple of dilutions without feeling constrained. Once set up correctly, it became a reliable part of our cytokine measurement toolkit.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.