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Macrophage Interleukin 10 (IL10) ELISA Kit, Colorimetric (MTS-1123-HM70)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for quantitative measurement of Interleukin 10 (IL10) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Analytical Method

Quantitative

Sample Type

Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Specificity

Interleukin 10 (IL10)

Specification

Size

96 tests

Sample Volume

100 μL

Assay Time

3 h

Plate

Pre-coated

Bioassay Target Name

Interleukin 10 (IL10)

Storage

4 °C, -20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

interleukin 10

Synonyms

CSIF; TGIF; GVHDS; IL-10; IL10A

Background

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM519	Rat		7.8 pg/mL-500 pg/mL	Inquiry
MTS-1123-HM520	Human		7.8 pg/mL-500 pg/mL	Inquiry
MTS-1123-HM521	Mouse		15.6-1000 pg/mL	Inquiry
MTS-1123-HM522	Rat		31.2-2000 pg/mL	Inquiry
MTS-1123-HM523	Mouse		7.8 pg/mL-500 pg/mL	Inquiry
MTS-1123-HM524	Pig		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM525	Human		12.5 pg/mL-800 pg/mL	Inquiry
MTS-1123-HM526	Chicken		7.8 pg/mL-500 pg/mL	Inquiry
MTS-1123-HM527	Rabbit		7.8 pg/mL-500 pg/mL	Inquiry
MTS-1123-HM528	Dog		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM529	Cow		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM530	Rat		6.25 pg/mL-400 pg/mL	Inquiry
MTS-1123-HM531	Human		3.12 pg/mL-200 pg/mL	Inquiry
MTS-1123-HM532	Camel		15.625 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM533	Sheep		15.625 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM534	Hamster		6.25 pg/mL-400 pg/mL	Inquiry
MTS-1123-HM535	Dog		7.8 pg/mL-500 pg/mL	Inquiry
MTS-1123-HM536	Mouse		1.56 pg/mL-100 pg/mL	Inquiry
MTS-1123-HM537	Goat		15.6-1000 pg/mL	Inquiry
MTS-1123-HM538	Horse		15.6-1000 pg/mL	Inquiry
MTS-1123-HM539	Monkey		15.6-1000 pg/mL	Inquiry
MTS-1123-HM540	Guinea Pig		3.12 pg/mL-200 pg/mL	Inquiry
MTS-1123-HM541	Golden Syrian Hamster		6.25 pg/mL-400 pg/mL	Inquiry
MTS-1123-HM542	Rhesus Monkey		15.6-1000 pg/mL	Inquiry

FAQs Customer Reviews Related Products

I'm measuring IL-10 from different matrices. What sample types does this kit support, and do I need different preparation steps for serum vs. tissue homogenate?

This is a sandwich, colorimetric, quantitative ELISA designed for common biological matrices including cell culture supernatant, plasma, serum, and tissue homogenate. For best accuracy, we recommend matrix-matching your standards when possible and using proper clarification (centrifugation/filtration) to reduce particulates-especially for tissue homogenates-so background and viscosity effects are minimized.

Is the plate pre-coated and what are the practical runtime and volume needs for a typical experiment day?

Yes, the kit uses a pre-coated plate, which shortens setup and improves consistency. The workflow typically takes about 3 hours from loading to reading, and the sample volume is 100 µL per well. This format is convenient for running a full standard curve plus duplicates, and it supports consistent inter-operator performance because coating variability is removed.

How should I store the kit and what is the shelf-life? I'm planning multiple small runs across a few months.

The kit supports storage at 4°C and -20°C (follow the protocol for component-specific handling), and the listed expiry is 6 months under recommended conditions. For multi-run planning, aliquot any reconstituted standards to avoid repeated freeze-thaw cycles, and keep reagents tightly sealed and protected from contamination to preserve signal stability across your study timeline.

Reliable quantitative IL‑10 results with low day-to-day variability
We used this colorimetric sandwich ELISA to quantify IL‑10 in macrophage-conditioned media and serum samples. The pre-coated plate made the workflow smooth, and the whole assay fit within an afternoon. The standard curve looked consistent across two operators, and we saw good separation between stimulated and control conditions without needing excessive troubleshooting. Packaging and component labeling were clear, which helped reduce setup errors.
Straightforward protocol and strong signal in culture supernatants
Our lab prioritized ease-of-use because we were screening many conditions. This kit's colorimetric readout was convenient and worked well for cell culture supernatants. The sample volume requirement was manageable, and the pre-coated format reduced variability compared with plates we used to coat ourselves. Turnaround time was fast enough to support weekly experiments and iterative optimization of macrophage polarization assays.
Good kit for multi-matrix IL‑10 quantification and planning
We ran plasma, serum, and tissue homogenate in parallel. The kit is positioned for multiple sample types, which matched our project needs. We appreciated the 6‑month expiry because we didn't need to rush all samples into one batch. Storage guidance was simple, and with careful sample clarification the background stayed controlled. Overall, it supported a practical multi-month study schedule.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.