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Macrophage Stimulating 1 Receptor ELISA Kit, qPCR (MTS-1123-HM45)

Overview

Description
Creative Biolabs provides sandwich ELISA kit for semi-quantitative measurement of Macrophage Stimulating 1 Receptor in different sample types by qPCR.
Applications
ELISA
Qualified With
Quality Certificate
Reactivity
Human
Detection Method
qPCR
Method Type
Sandwich ELISA
Analytical Method
Semi-Quantitative
Sample Type
Cell Culture Supernatant, Plasma, Serum
Specificity
Macrophage Stimulating 1 Receptor

Specification

Size
96 tests
Sample Volume
25 µL
Plate
Pre-coated
Bioassay Target Name
Macrophage Stimulating 1 Receptor
Storage
4 °C, -20 °C, -80 °C
Storage Comment
Reference to the protocol
Expiry Date
6 months
Product Disclaimer
This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name
macrophage stimulating 1 receptor
Synonyms
RON; SEA; PTK8; CD136; NPCA3; CDw136; p185-Ron
Background
This gene encodes a cell surface receptor for macrophage-stimulating protein (MSP) with tyrosine kinase activity. The mature form of this protein is a heterodimer of disulfide-linked alpha and beta subunits, generated by proteolytic cleavage of a single-chain precursor. The beta subunit undergoes tyrosine phosphorylation upon stimulation by MSP. This protein is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung, and together with MSP, thought to be involved in host defense. Alternative splicing generates multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing.
FAQs Customer Reviews Related Products

The listing says "sandwich ELISA" with "qPCR" readout and "semi-quantitative." How should we plan experiments so reviewers won't question quantification rigor?

This kit is positioned for semi-quantitative measurement of Macrophage Stimulating 1 Receptor using a sandwich ELISA format with a qPCR-based readout. To support rigor, plan for relative comparisons (fold change vs control), include replicate controls, and document curve behavior and run acceptance criteria. If your publication requires absolute quantification, consider pairing results with an orthogonal method. We can advise on positioning and data presentation so the methodology matches your claims and the conclusions remain defensible.

If I'm already doing gene-expression qPCR, does this kit help, or is it redundant?

It can be complementary rather than redundant. Gene-expression qPCR measures transcript abundance, while this kit is intended to measure the receptor at the protein level, which may not correlate perfectly due to translation, trafficking, and turnover. Many customers use transcript qPCR for mechanism and the protein readout for functional relevance. If you're investigating receptor modulation, protein-level confirmation often strengthens conclusions, especially when treatments affect post-transcriptional regulation.

What are your best practices to reduce run-to-run variability in amplification-based readouts?

Consistency is key: align pipetting technique, keep incubation timing stable, and standardize plate handling to minimize edge effects. Use the same operator and calibrated equipment when possible. Because the kit is semi-quantitative across different sample types, we strongly recommend including internal reference samples (a pooled control) on every plate to normalize across runs. If you share your workflow and throughput needs, we can suggest a plate map strategy and QC checkpoints to keep data comparable over time.

  • Useful protein-level confirmation alongside transcript qPCR studies for macrophage research workflows
    We were already measuring MS1R transcripts and needed a protein-level layer to strengthen our conclusions. This qPCR-readout ELISA format helped us compare treatment groups consistently and supported clear relative changes across conditions. We treated it as semi-quantitative, which matched our goals for screening and prioritization. Vendor support was helpful in recommending internal reference samples to stabilize cross-run comparisons. Overall, it added value beyond transcript-only analysis.
  • Good semi-quantitative performance for condition-to-condition comparisons in our inflammation assay pipeline
    We used the kit to compare receptor changes under several stimulation conditions. The workflow was manageable, and results were consistent with expected directionality from our pathway biology. We appreciated the supplier's clarity that the method is semi-quantitative, which helped us frame our analysis appropriately. After adopting recommended controls and consistent timing, the data were reproducible and fit our decision-making needs for follow-up experiments.
  • Helpful guidance on experimental design and data presentation for routine lab and screening use
    Our biggest concern was how to present semi-quantitative results in a way that would stand up to internal review. Support provided a sensible plan: replicate controls, defined acceptance criteria, and normalization using a pooled reference sample. The assay helped us rank conditions and select a few for deeper validation. We found it practical and consistent, especially when used as designed for relative comparisons rather than absolute quantification.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.

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