This kit is positioned for semi-quantitative measurement of Macrophage Stimulating 1 Receptor using a sandwich ELISA format with a qPCR-based readout. To support rigor, plan for relative comparisons (fold change vs control), include replicate controls, and document curve behavior and run acceptance criteria. If your publication requires absolute quantification, consider pairing results with an orthogonal method. We can advise on positioning and data presentation so the methodology matches your claims and the conclusions remain defensible.
It can be complementary rather than redundant. Gene-expression qPCR measures transcript abundance, while this kit is intended to measure the receptor at the protein level, which may not correlate perfectly due to translation, trafficking, and turnover. Many customers use transcript qPCR for mechanism and the protein readout for functional relevance. If you're investigating receptor modulation, protein-level confirmation often strengthens conclusions, especially when treatments affect post-transcriptional regulation.
Consistency is key: align pipetting technique, keep incubation timing stable, and standardize plate handling to minimize edge effects. Use the same operator and calibrated equipment when possible. Because the kit is semi-quantitative across different sample types, we strongly recommend including internal reference samples (a pooled control) on every plate to normalize across runs. If you share your workflow and throughput needs, we can suggest a plate map strategy and QC checkpoints to keep data comparable over time.
For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.