Macrophage Chemotactic Factor (MCF) ELISA Kit, Colorimetric (MTS-1123-HM52)

Yes-colorimetric ELISA is designed for common microplate readers typically configured around 450 nm. We recommend confirming your instrument's filters and ensuring linear absorbance in your expected range. If multiple users share the reader, standardized read settings and timing matter. We can share best practices for consistent stop-solution timing and plate handling to reduce variability between operators.

You can, but supplement-heavy media can increase background or interfere with antibody binding. We suggest validating with spiked standards in your exact medium and performing dilution linearity checks. If background rises, diluting samples, clarifying by centrifugation, and using matched blank media controls typically helps. These steps ensure the signal reflects MCF rather than media artifacts.

For cross-plate comparisons, include a bridging control sample (e.g., pooled supernatant) on every plate and normalize group signals relative to that control. Keep incubation times consistent, avoid mixing lots mid-study, and use the same curve-fitting approach. If you must change lots, we recommend running an overlap plate with both lots to quantify any shift before continuing.