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Macrophage Chemokine Ligand 26 (CCL26) ELISA Kit, Colorimetric (MTS-1123-HM36)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for quantitative measurement of Chemokine Ligand 26 (CCL26) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Analytical Method

Quantitative

Sample Type

Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Specificity

Chemokine Ligand 26 (CCL26)

Specification

Size

96 tests

Sample Volume

100 μL

Assay Time

3 h

Plate

Pre-coated

Bioassay Target Name

Chemokine Ligand 26 (CCL26)

Storage

4 °C, -20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

C-C motif chemokine ligand 26

Synonyms

IMAC; TSC-1; MIP-4a; SCYA26; MIP-4alpha

Background

This gene is one of two Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 7. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for normal peripheral blood eosinophils and basophils. This protein also has antimicrobial activity, displaying an antibacterial effect on S. pneumoniae, S. aureus, Non-typeable H. influenzae, and P. aeruginosa. The product of this gene is one of three related chemokines that specifically activate chemokine receptor CCR3. This chemokine may contribute to the eosinophil accumulation in atopic diseases.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM308	Human		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM309	Mouse		6.25 pg/mL-400 pg/mL	Inquiry
MTS-1123-HM310	Dog		User optimized	Inquiry

FAQs Customer Reviews Related Products

Is the colorimetric CCL26 kit intended for quantitative measurement, and what ELISA format is used?

Based on how this product line is described across related listings, the colorimetric CCL26 kit is aligned with a colorimetric ELISA workflow for measuring CCL26; in this catalog, colorimetric kits are typically positioned for quantitative measurement in a standard ELISA application context. If you need strict claims for a manuscript, we recommend validating linearity in your own matrix via dilution series and running a full standard curve each time to support quantitation and reproducibility.

I'm comparing CCL26 in serum vs. plasma-what's your recommendation to avoid misleading differences?

Serum and plasma can differ due to clotting-related release, anticoagulant effects, and protein binding. To reduce misleading shifts, use consistent collection tubes, process samples quickly, and avoid repeated freeze-thaw cycles. Run matched dilutions (at least two) for both matrices and confirm recovery with a spike test if possible. If your end goal is ranking samples rather than absolute values, keep matrix type consistent within each comparison group.

What's a sensible approach if my CCL26 levels are near the detection limit?

For low-abundance targets, the most effective approach is a pilot that tests larger effective input (if permitted), minimal dilution, and careful background control. Run duplicates or triplicates, and confirm that your blank and low standard points are stable. If your matrix suppresses signal, consider using a cleaner sample preparation or switching to a detection approach with higher sensitivity if available. In all cases, consistent handling and curve quality are key.

Practical colorimetric workflow for routine CCL26 monitoring in human samples
We needed a straightforward CCL26 assay that our team could run routinely without specialized equipment. The colorimetric format fits our standard plate reader workflow and integrates well into our cytokine panel schedule. After running a dilution pilot for serum, we achieved stable readings and good separation between cohorts. For labs doing routine monitoring and comparative analysis, this kit style is a practical choice with minimal friction.
Good consistency across runs once sample handling and dilution were standardized
Our first run taught us that matrix and dilution choice mattered a lot for CCL26. Once we standardized serum processing time and selected a consistent dilution factor, the results became very repeatable between plates. We also included a pooled reference sample to track variability. This made it easier to compare different experimental batches and confidently interpret trends without re-running plates due to out-of-range readings.
Solid choice for comparative studies, especially when paired with a reference control
We used the kit for comparing CCL26 across treatment groups and donors, and it performed well for relative differences. The biggest improvement came from keeping a frozen aliquoted reference control on every plate, which helped normalize small day-to-day variation. While we didn't push the assay to extremes of sensitivity, it worked reliably within our expected biological range and produced data we could confidently analyze.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.