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Macrophage Chemotactic Factor (MCF) Competition ELISA Kit (MTS-1123-HM53)

Datasheet

Overview

Description

Creative Biolabs provides competition ELISA kit for quantitative measurement of Macrophage Chemotactic Factor (MCF) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Reactivity

Human

Detection Method

Colorimetric

Method Type

Competition ELISA

Analytical Method

Quantitative

Sensitivity

1.0 pg/mL

Sample Type

Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Specificity

Macrophage Chemotactic Factor (MCF)

Specification

Size

96 tests

Detection Range

50-1000 pg/mL

Sample Volume

100 μL

Assay Time

1.5 h

Plate

Pre-coated

Bioassay Target Name

Macrophage Chemotactic Factor (MCF)

Storage

4 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

FAQs Customer Reviews Related Products

Why choose a competitive MCF kit instead of the sandwich version?

Competitive ELISA is a good option when sample concentrations vary widely, when epitope accessibility may change, or when the target/antibody pairing is better suited to a competition format. If you expect strong matrix effects or uncertain abundance, competitive assays can be more forgiving after you establish a working dilution. We can help you decide based on your sample type, expected range, and required sensitivity.

Can competitive assays still deliver reproducible quantification for publication figures?

Yes, provided you standardize key variables. We recommend running standards and controls in duplicates or triplicates, keeping timing consistent, and using the same dilution factor across comparable groups. Including an internal QC sample on each plate is particularly helpful for competitive ELISA to monitor drift. With disciplined execution and basic validation (recovery/linearity), the output is suitable for publication-quality comparisons.

What are common causes of low signal or compressed curves in competition ELISA?

The most common causes are incorrect dilution (too concentrated or too dilute), insufficient mixing, shortened incubation, or suboptimal washing. Because signal is inversely related to analyte concentration, it can feel counterintuitive at first. We suggest running a small dilution series, ensuring thorough mixing of standards/samples, and keeping incubation uniform across wells. If needed, we can review your plate map and workflow.

Competitive format handled our variable MCF samples surprisingly well
Our MCF levels were unpredictable across donors and stimulation conditions, so we preferred a competitive format. After a quick dilution pilot, results became stable and comparable. The protocol was clear, and we liked using an internal pooled control to track day-to-day variation. It gave us confidence in our comparative conclusions without forcing constant re-optimization.
Good troubleshooting support for inverse-signal interpretation and QC
Our team initially misread the competition curve direction, but support clarified the logic and suggested better control placement. Once we adjusted the plate layout and dilution strategy, curves looked cleaner and replicates tightened. The assay became a dependable tool for ranking conditions and selecting candidates for deeper functional testing, which was our main goal.
Works well with strict timing; helped us reduce repeat experiments
We found that consistent incubation and wash discipline were the keys. With those controls in place, the kit produced repeatable curves across multiple plates. We used it to screen many samples rapidly and narrowed our follow-up set significantly. It's a solid option when you need comparative quantification across a wide range and want to avoid constant reruns.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.