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Macrophage Chemokine Ligand 2 (CXCL2) ELISA Kit, qPCR (MTS-1123-HM40)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for semi-quantitative measurement of Chemokine Ligand 2 (CXCL2) in different sample types by qPCR.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

qPCR

Method Type

Sandwich ELISA

Analytical Method

Semi-Quantitative

Sample Type

Cell Culture Supernatant, Plasma, Serum

Specificity

Chemokine Ligand 2 (CXCL2)

Specification

Size

96 tests

Sample Volume

25 µL

Plate

Pre-coated

Bioassay Target Name

Chemokine Ligand 2 (CXCL2)

Storage

4 °C, -20 °C, -80 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

C-X-C motif chemokine ligand 2

Synonyms

GRO2; GROb; MIP2; MIP2A; SCYB2; MGSA-b; MIP-2a; CINC-2a

Background

This antimicrobial gene is part of a chemokine superfamily that encodes secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CXC subfamily, is expressed at sites of inflammation and may suppress hematopoietic progenitor cell proliferation.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM817	Mouse	1 pg/mL		Inquiry
MTS-1123-HM818	Rat	0.2 pg/mL		Inquiry

FAQs Customer Reviews Related Products

I'm debating between a classic colorimetric ELISA and this qPCR-based readout. What is the real benefit, and when should I choose this kit?

This kit is designed for semi-quantitative CXCL2 measurement using a sandwich ELISA format with qPCR readout. Customers typically choose it when they want stronger signal discrimination in challenging matrices, or when sample availability is limited and they need a readout that can extend analytical flexibility. If your lab already has a validated qPCR workflow and you want consistent amplification-based detection, this format can be a practical fit.

How do you recommend handling samples with potential inhibitors (e.g., complex lysates or media additives) to avoid inconsistent results?

For complex matrices, we recommend using a clean sample preparation workflow, minimizing harsh detergents, and running a small pilot dilution series to identify the dilution that balances detectability with matrix effects. Because this kit is designed for use across different sample types, a pre-test is the fastest way to find your optimal condition. Our technical team can also advise based on your buffer composition, blockers, and the likely inhibitor sources.

What documentation and support do you provide so we can validate the assay quickly for publication-quality data?

Each kit is supported by a datasheet and QC-related documentation, and our support team can help you design validation steps such as spike-and-recovery, dilution linearity, and inter-assay reproducibility planning. We can also recommend control strategies (positive/negative samples, blank controls, and curve design) to match your target biology. If you share your species, matrix, and expected CXCL2 range, we'll suggest a practical validation plan to accelerate readiness.

Helpful semi-quantitative workflow with strong technical support for macrophage research workflows
We used this CXCL2 kit to compare inflammatory conditions across multiple macrophage stimulation groups. The qPCR-based readout fit our lab setup well and helped us keep the workflow consistent with our existing amplification equipment. The supplier's guidance on pilot dilution testing and control selection saved time when we encountered matrix effects from conditioned media. Overall, it performed reliably for trend comparisons and reduced back-and-forth during assay setup.
Good option when colorimetric signals are hard to separate in our inflammation assay pipeline
Our previous assays showed overlapping signals in complex samples, so we tried the qPCR format to improve discrimination between moderate changes. The kit instructions were straightforward, and the support team answered practical questions about sample handling and plate organization. While we still did an internal validation, the process was smooth and the results aligned with expected biology. It's a solid choice for semi-quantitative studies in demanding matrices.
Smooth ordering, clear documentation, and consistent run-to-run results for routine lab and screening use
We ran several plates across different days and saw consistent trends across replicates. The datasheet overview clarified the kit's intent as semi-quantitative, which matched our experimental goal for screening rather than absolute quantification. The supplier was responsive on shipping and also provided troubleshooting suggestions for one plate that had edge effects. We appreciated the practical, lab-friendly advice and the reproducibility across repeats.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.