Macrophage Colony Stimulating Factor 1 (M-CSF/CSF1) Competition ELISA Kit (MTS-1123-HM59)

Competitive ELISA can be a strong choice when concentrations may be high or uncertain, because the format can remain interpretable after you establish the right dilution factor. We recommend running a pilot dilution curve to identify a stable operating range, then applying that dilution consistently across comparable samples. This approach reduces repeats, avoids saturation issues, and supports cleaner comparisons across groups.

Common criteria include consistent standard curve shape, replicate agreement (low CV), and stable readings of a QC sample repeated on each plate. We also suggest checking dilution linearity for representative samples and confirming spike recovery in your matrix. Establish these benchmarks early, then apply them across the study so any drift or workflow error is detected quickly before it affects many plates.

Yes, that's a smart workflow. Competitive ELISA can efficiently rank or screen samples across conditions. Many labs then confirm a focused subset using an orthogonal method (another immunoassay format or transcript data) to strengthen conclusions. We recommend including consistent controls so your screening data remain comparable across plates, making it easier to select a representative confirmation set.