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Macrophage Interleukin 6 (IL6) ELISA Kit, Colorimetric (MTS-1123-HM73)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for quantitative measurement of Interleukin 6 (IL6) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Analytical Method

Quantitative

Sample Type

Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Urine

Specificity

Interleukin 6 (IL6)

Specification

Size

96 tests

Sample Volume

100 μL

Assay Time

1 - 4.5 h

Plate

Pre-coated

Bioassay Target Name

Interleukin 6 (IL6)

Storage

4 °C, -20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

interleukin 6

Synonyms

CDF; HGF; HSF; BSF2; IL-6; BSF-2; IFNB2; IFN-beta-2

Background

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2).

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM552	Human		7.8-500 pg/mL	Inquiry
MTS-1123-HM553	Mouse		7.8-500 pg/mL	Inquiry
MTS-1123-HM554	Rat		7.8-500 pg/mL	Inquiry
MTS-1123-HM555	Dog		7.8-500 pg/mL	Inquiry
MTS-1123-HM556	Rabbit		3.12 pg/mL-200 pg/mL	Inquiry
MTS-1123-HM557	Cow		7.8-500 pg/mL	Inquiry
MTS-1123-HM558	Pig		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM559	Chicken		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM560	Guinea Pig		7.8-500 pg/mL	Inquiry
MTS-1123-HM561	Horse		7.8-500 pg/mL	Inquiry
MTS-1123-HM562	Monkey		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM563	Golden Syrian Hamster		7.8-500 pg/mL	Inquiry
MTS-1123-HM564	Cow		1.56 pg/mL-100 pg/mL	Inquiry
MTS-1123-HM565	Rat		1.56 pg/mL-100 pg/mL	Inquiry
MTS-1123-HM566	Human		0.78 pg/mL-50 pg/mL	Inquiry
MTS-1123-HM567	Mouse		1.56 pg/mL-100 pg/mL	Inquiry
MTS-1123-HM568	Goat		15.62 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM569	Goat		0.312 pg/mL-20 pg/mL	Inquiry
MTS-1123-HM570	Sheep		1.56 pg/mL-100 pg/mL	Inquiry
MTS-1123-HM571	Rhesus Monkey		40-10000 pg/mL	Inquiry

FAQs Customer Reviews Related Products

We test IL‑6 in urine and tissue homogenates as well as serum. Is this kit suitable for that mix of samples?

Yes. This kit is a sandwich ELISA, colorimetric, and quantitative, and lists sample compatibility including serum, plasma, cell culture supernatant, tissue homogenate, and urine. For best results, treat each matrix carefully (clarify particulates, avoid hemolysis, standardize dilution), and consider running matrix-specific dilution checks to ensure your samples fall within the assay's usable signal range.

What is the runtime and per-well sample volume? We're planning a high-throughput day.

The kit uses a pre-coated plate, requires 100 µL sample volume, and the reported assay time is 1-4.5 hours depending on protocol choices. This flexibility is helpful when scheduling multiple plates. If throughput is the priority, set up consistent incubation timing across plates, prepare wash buffers in advance, and run standards/controls on every plate to maintain batch comparability.

How do you recommend storing components between partial runs, and what is the expiry?

Storage conditions are listed as 4°C and -20°C, with an expiry date of 6 months. For partial runs, avoid repeated freeze-thaw of sensitive reagents: aliquot reconstituted standards, keep detection reagents protected from contamination, and seal unused strips/plates tightly. These practices typically preserve performance when you spread experiments across weeks instead of running all 96 tests at once.

Broad sample compatibility made IL‑6 studies much easier to manage
We needed one IL‑6 kit that could handle urine plus tissue homogenate and serum. This kit's stated sample types matched our requirements, and the quantitative colorimetric readout was straightforward. The pre-coated plate saved time, and once we standardized dilution factors for each matrix, results were consistent. It streamlined our workflow because we didn't have to validate multiple kits for different sample sources.
Fast setup, flexible runtime, and dependable curve behavior
The variable assay time (up to about 4.5 hours) helped us fit the assay around cell culture timing. We appreciated the pre-coated format and the 100 µL volume, which worked well with our sample availability. Standard curves were stable across plates, and controls behaved predictably. Overall, it was a practical quantitative ELISA for routine macrophage inflammation experiments.
Good kit for repeated runs across months with proper storage habits
Because our project was long, we valued the 6‑month expiry and dual storage options. We ran partial plates over time and maintained performance by aliquoting standards and minimizing freeze-thaw. The assay was reproducible and helped us monitor IL‑6 shifts after different stimuli. For labs managing staggered sample collection, it supports a realistic, phased testing plan.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.