Macrophage Chemokine Ligand 9 (CXCL9) ELISA Kit, qPCR (MTS-1123-HM112)

In this format, the target is still captured by immuno‑binding on a plate (like a traditional ELISA), but the detection signal is amplified and read out via qPCR rather than enzyme‑substrate color development. This can improve sensitivity and help detect very low amounts of CXCL9, which is valuable for limited‑volume samples or early‑timepoint macrophage studies where secretion is subtle. Many users choose qPCR‑based immunoassays when conventional colorimetric ELISA signals sit close to background or when they need stronger discrimination between low‑expressing groups. Because the readout is qPCR, careful control of amplification conditions and contamination prevention is important, and results are typically treated as semi‑quantitative unless a robust standardization strategy is applied. If you have access to a qPCR instrument and need enhanced sensitivity, this option is often a strong fit.

This qPCR‑readout immunoassay is designed to work with smaller input volumes than many classic ELISAs, which can be helpful when sample availability is constrained. To maximize repeatability, standardize sample collection and storage (aliquot, snap‑freeze, avoid multiple freeze-thaws), and keep all plates and reagents at consistent temperatures during the binding steps. On the qPCR side, use validated pipettes, consistent cycling parameters, and include no‑template controls and positive controls on every run to detect contamination or reagent drift. If your lab runs multiple qPCR platforms, stick to one instrument for a study to reduce inter‑instrument variability. Finally, ensure that washing is thorough between binding steps, because residual components can affect downstream amplification and increase Ct variability.

CXCL9 kits are commonly offered in species‑specific configurations because antibody affinity and epitope conservation can differ between organisms. To confirm suitability, match the kit's stated species to your model (for example, human vs. mouse) and review any provided cross‑reactivity or validation notes. If you are working with less common species, we recommend contacting support with your organism and sample type so we can verify the appropriate variant and expected performance. In experimental design, include matrix‑matched controls (e.g., mouse serum standards if available) and run pilot samples to confirm that Ct values fall within the assay's effective range. If you observe unexpected signals, evaluate sample dilution, potential heterophilic antibody interference, and the consistency of your qPCR workflow before drawing biological conclusions.