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Macrophage TGF-beta (TGFβ) ELISA Kit, Colorimetric (MTS-1123-HM102)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for quantitative measurement of TGF-beta (TGFβ) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Analytical Method

Quantitative

Sample Type

Plasma, Serum, Tissue Homogenate

Specificity

TGF-beta (TGFβ)

Specification

Size

96 tests

Sample Volume

100 μL

Plate

Pre-coated

Bioassay Target Name

TGF-beta (TGFβ)

Storage

4 °C, -20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM706	Hamster		31.25 pg/mL-2000 pg/mL	Inquiry
MTS-1123-HM707	Cow		31.25 pg/mL-2000 pg/mL	Inquiry
MTS-1123-HM708	Dog		50-1000 pg/mL	Inquiry

FAQs Customer Reviews Related Products

Is sample activation required before TGF-beta detection?

TGF-beta is often present in latent form in biological samples. This kit includes guidance for acid activation to release active TGF-beta prior to measurement. Proper activation ensures accurate quantification. The optimized protocol maintains linearity and reproducibility after activation, supporting serum, plasma, and culture supernatant analysis.

Does the colorimetric system provide sufficient sensitivity?

The optimized antibody pairing and TMB detection system provide strong sensitivity suitable for both basal and induced TGF-beta levels. Background signal is minimized through validated blocking conditions, ensuring reliable absorbance readings.

How consistent is the assay performance?

Quality validation ensures low intra- and inter-assay variation. When incubation timing and washing steps are carefully controlled, the assay produces stable standard curves and highly consistent replicate data.

Stable and reproducible TGF-beta quantification
We used this kit in macrophage polarization experiments and obtained consistent TGF-beta measurements. Acid activation was straightforward, and the standard curve demonstrated excellent linearity. Replicate wells showed minimal variability.
Reliable for fibrosis-related research
In a fibrosis-associated macrophage model, this kit provided clear differentiation between control and treated groups. Background signal remained low, and the assay workflow was manageable within a standard laboratory schedule.
Good batch consistency
We tested two independent batches and observed minimal differences in curve shape and absorbance values. This reliability is critical for longitudinal studies.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.