Macrophage Interleukin 2 (IL2) ELISA Kit, qPCR (MTS-1123-HM69)

Many teams use this kit in workflows where secreted cytokine changes are interpreted alongside transcriptional signatures, especially in mixed immune models. IL-2 is often the functional output of activated lymphocytes, while macrophages can modulate that activation state. We recommend designing your experiment so secretion and gene expression time points are coordinated, enabling a clear link between IL-2 output and pathway activation rather than treating them as separate datasets.

Yes-fold-change comparisons are often the most robust way to report IL-2 in complex immune models. Absolute IL-2 concentrations can be influenced by donor variability, co-culture ratios, and timing. If you standardize cell numbers, stimulation duration, and sample dilution, fold-change results become very stable and interpretable. Adding an internal reference sample across plates further improves confidence that observed changes are biological rather than procedural.

We suggest a shared SOP with fixed incubation windows, a standardized wash method, and a single agreed-upon dilution strategy. Include duplicates (or triplicates for key samples), and use a common internal control sample on every plate. Also ensure everyone uses the same plate reader settings and curve-fitting approach. These operational controls typically reduce inter-operator variability more effectively than any single reagent tweak.