Online Inquiry

Macrophage Chemokine Ligand 16 (CXCL16) ELISA Kit, qPCR (MTS-1123-HM120)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for semi-quantitative measurement of Chemokine Ligand 16 (CXCL16) in different sample types by qPCR.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

qPCR

Method Type

Sandwich ELISA

Analytical Method

Semi-Quantitative

Sample Type

Cell Culture Supernatant, Plasma, Serum

Specificity

Chemokine Ligand 16 (CXCL16)

Specification

Size

96 tests

Sample Volume

25 µL

Plate

Pre-coated

Bioassay Target Name

Chemokine Ligand 16 (CXCL16)

Storage

4 °C, -20 °C, -80 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

C-X-C motif chemokine ligand 16

Synonyms

SRPSOX; CXCLG16; SR-PSOX

Background

Enables chemokine activity. Involved in several processes, including positive regulation of cell growth; response to interferon-gamma; and response to tumor necrosis factor. Located in extracellular space. Biomarker of COVID-19 and systemic scleroderma.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM925	Mouse	0.13 pg/mL		Inquiry
MTS-1123-HM926	Human	0.3 pg/mL		Inquiry

FAQs Customer Reviews Related Products

We're deciding between measuring CXCL16 protein by ELISA vs. CXCL16 mRNA by qPCR. When is ELISA the better choice, and do you have recommended pairing strategies?

ELISA is the better choice when your biological question is about secreted/soluble CXCL16 protein abundance, functional ligand availability, or comparing release across stimuli, donors, or treatments-because protein levels can diverge from mRNA due to secretion dynamics and post-transcriptional regulation. A strong pairing strategy is to collect matched samples: harvest RNA at an early time point (to capture transcriptional changes) and supernatant at later time points (to capture accumulated secretion). If you anticipate shedding/processing, protein readouts may be essential even when mRNA changes look modest.

I'm measuring CXCL16 in macrophage-related samples. What sample types does this kit support, and how should I choose dilution factors to stay within a reliable signal window?

This kit is positioned for sandwich ELISA-based, semi-quantitative CXCL16 measurement across different sample types, with the workflow guided by the provided protocol. In practice, we recommend running a small pilot with 2-3 serial dilutions (e.g., 1:2, 1:5, 1:10) for each matrix (cell culture supernatant vs. lysate vs. serum/plasma if applicable) to identify a dilution that yields a clean standard-like curve shape, acceptable background, and falls within the mid-range of the standard curve. If your matrix has high protein or detergent content, consider additional dilution or buffer exchange to reduce matrix effects and improve precision.

Clear protocol and stable signal for stimulated macrophage supernatants
We used this CXCL16 kit to compare chemokine release after Toll-like receptor stimulation in macrophage cultures. The workflow felt straightforward and the plate readouts were consistent when we standardized incubation timing and washing. The biggest benefit was getting a protein-level answer that matched our functional hypothesis better than transcript data alone. Background was manageable after we optimized sample dilution, and the standard curve behaved predictably across repeats. For multi-condition screening, it saved time versus developing an in-house ELISA and gave us confidence in trend interpretation.
Helpful for time-course studies where protein secretion lags behind mRNA
Our project required measuring CXCL16 over several time points to capture secretion kinetics. The kit worked well once we set up a small pilot dilution series and created a single internal control aliquot to run on every plate. That internal control approach made it easy to spot day-to-day drift and keep results comparable across batches. We appreciated that the assay supported semi-quantitative comparisons across different sample types, which fit our exploratory stage. Shipping and storage planning were also easy once we aligned our run schedule with reagent handling.
Good fit for screening conditions before moving to targeted validation assays
We bought this kit as a screening tool to identify which macrophage polarization conditions produced higher CXCL16 signals. It performed reliably enough to rank conditions and select a smaller set for deeper follow-up (flow cytometry and pathway assays). The main learning was to treat each matrix carefully: supernatants behaved differently than lysates, so dilution optimization mattered. Once set, replicate CVs were acceptable and the assay was easy to repeat. Overall, it's a practical option when you need actionable protein trends quickly.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.