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Macrophage Chemokine Ligand 2 (CCL2) Competition ELISA Kit (MTS-1123-HM83)

Datasheet

Overview

Description

Creative Biolabs provides competition ELISA kit for quantitative measurement of Chemokine Ligand 2 (CCL2) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Competition ELISA

Analytical Method

Quantitative

Sample Type

Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Specificity

Chemokine Ligand 2 (CCL2)

Specification

Size

96 tests

Sample Volume

100 μL

Assay Time

1.5 h

Plate

Strips (12 x 8)

Bioassay Target Name

Chemokine Ligand 2 (CCL2)

Storage

4 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

C-C motif chemokine ligand 2

Synonyms

HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30

Background

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM635	Goat		User optimized	Inquiry
MTS-1123-HM636	Cow		0.5-10 ng/mL	Inquiry
MTS-1123-HM637	Human		100-2500 pg/mL	Inquiry
MTS-1123-HM638	Sheep		0.5-10 ng/mL	Inquiry
MTS-1123-HM639	Chicken		User optimized	Inquiry
MTS-1123-HM640	Rabbit		0.5-10 ng/mL	Inquiry
MTS-1123-HM641	Guinea Pig		0.5-10 ng/mL	Inquiry
MTS-1123-HM642	Rat		50-1000 pg/mL	Inquiry
MTS-1123-HM643	Mouse		50-1000 pg/mL	Inquiry
MTS-1123-HM644	Mouse		7.8-500 pg/mL	Inquiry

FAQs Customer Reviews Related Products

Why would I choose a competition ELISA for CCL2 instead of a sandwich ELISA?

A competition ELISA can be advantageous when sample conditions or analyte characteristics make sandwich binding less optimal. This kit is specified as a competition ELISA, colorimetric, and quantitative, with sample types including cell culture supernatant, plasma, serum, and tissue homogenate. If you are comparing complex matrices, competition formats may help manage certain interferences-but you should still validate dilution linearity and recovery in your matrix.

How long does the assay take, and what's the typical sample volume per well?

The listed assay time is ~1.5 hours with 100 μL sample volume per well, and the kit size is 96 tests. Plates are provided as strips (12×8), which is convenient when you don't want to run a full plate at once. Storage is listed at 4°C with a stated 6-month expiry, so planning aliquots and scheduling runs can reduce waste.

Can I run both serum and macrophage supernatants in the same plate?

You can, but only if you treat them as distinct matrices during validation. Since serum and culture media differ significantly, we recommend separate dilution series and spike-recovery checks for each matrix, even on the same plate. Use matrix-matched blanks, keep incubation timing identical, and consider placing matrices in separate plate regions to reduce handling mistakes. This approach lets you compare within each matrix confidently and avoid false differences caused by matrix effects.

Fast protocol and flexible strip plate format made planning easier
The strip plate layout was a major convenience for our macrophage project because we didn't always have 96 wells worth of samples at once. The assay time was short enough to fit into a busy day, and after we validated dilution factors, the data were consistent. We also appreciated that the kit supports multiple sample types, which helped us test both cell culture supernatants and a small serum set. Turnaround was efficient and re-runs were rare.
Competition format handled our complex tissue homogenate samples well
We measured CCL2 in tissue homogenates and had issues with other assays in the past. This competition kit produced more stable behavior once we optimized homogenate dilution and clarified samples thoroughly. The workflow was manageable and the results aligned with our immunology readouts. It's not a magic solution-you still need good controls-but it supported our in vivo study better than expected.
Good quantitative performance after standardizing wash and timing steps
Our first run showed us that strict timing and consistent washing really matter. After standardizing those steps and running duplicates, the assay gave reliable quantitation across our macrophage stimulation conditions. We liked the short turnaround and the fact that storage requirements were simple. Overall, it became a dependable part of our chemokine measurement toolkit.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.