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Ex Vivo Human M1 Macrophage Differentiation Kit (MTS-0124-WR4)

Datasheet

Overview

Description

The kit allows for the differentiation of human CD14+ monocytes into M1 macrophages. These activated M1 macrophages express high levels of IL-12 and low levels of IL-10, indicating a pro-inflammatory phenotype.

Applications

The kit is used to analyze the differentiation of human monocytes into M1 macrophages ex vivo.

Reactivity

Human

Detection Method

Flow cytometry

Method Type

Fluorochrome

Specification

Size

1 EA

Bioassay Target Name

CD14; CD80; CD163; CD206; IL-10; IL-12

Handling Advice

Ensure personal protection by wearing gloves, protective clothing, and eye/face gear. Practice proper hand hygiene after handling.

Storage

Stort the unopened product at -20 to -70 °C.

Storage Comment

Upon receipt, please store at the appropriate condition as directed.

Note

Store in a manual defrost freezer and avoid multiple freeze-thaw cycles.

Shipping

Dry ice

Target Details

Background

Differentiation of monocytes into M1 and M2 macrophages can be achieved in vitro using GM-CSF or M-CSF, respectively. These distinct subtypes can be identified by their unique cell-surface protein expression and production of pro- or anti-inflammatory cytokines.

FAQs Customer Reviews Related Products

Can this kit reliably generate functional M1 macrophages?

Yes. The kit supports differentiation of CD14+ monocytes into classically activated M1 macrophages with characteristic cytokine and surface marker profiles.

What starting material is recommended?

Purified human CD14+ monocytes from peripheral blood are recommended for optimal differentiation efficiency.

How should differentiation be validated?

Validation can be performed via flow cytometry for surface markers and ELISA or qPCR for cytokine expression analysis.

Consistent Polarization
This kit enabled consistent generation of M1 macrophages with strong pro-inflammatory characteristics. Cytokine analysis confirmed elevated IL-12 expression and reproducibility was significantly improved over in-house protocols.
User-Friendly Kit
The differentiation process was straightforward and reliable. The resulting macrophages displayed expected activation markers, reducing variability in our experiments.
Reliable Performance
Switching to this kit improved consistency in our polarization assays. Instructions were clear and validation confirmed the expected M1 phenotype.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.