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Ex Vivo Human M1 Macrophage Differentiation Kit (MTS-0124-WR4)

Datasheet

Overview

Description

The kit allows for the differentiation of human CD14+ monocytes into M1 macrophages. These activated M1 macrophages express high levels of IL-12 and low levels of IL-10, indicating a pro-inflammatory phenotype.

Applications

The kit is used to analyze the differentiation of human monocytes into M1 macrophages ex vivo.

Reactivity

Human

Detection Method

Flow cytometry

Method Type

Fluorochrome

Specification

Size

1 EA

Bioassay Target Name

CD14; CD80; CD163; CD206; IL-10; IL-12

Handling Advice

Ensure personal protection by wearing gloves, protective clothing, and eye/face gear. Practice proper hand hygiene after handling.

Storage

Stort the unopened product at -20 to -70 °C.

Storage Comment

Upon receipt, please store at the appropriate condition as directed.

Note

Store in a manual defrost freezer and avoid multiple freeze-thaw cycles.

Shipping

Dry ice

Target Details

Background

Differentiation of monocytes into M1 and M2 macrophages can be achieved in vitro using GM-CSF or M-CSF, respectively. These distinct subtypes can be identified by their unique cell-surface protein expression and production of pro- or anti-inflammatory cytokines.