Macrophage Migration Inhibitory Factor (MIF) ELISA Kit, qPCR (MTS-1123-HM51)

This product is positioned as a sandwich ELISA solution with qPCR-labeled characterization/compatibility on the page, so we recommend treating it as an ELISA-driven quantification workflow while using qPCR as an orthogonal confirmation method if needed. Many labs pair protein-level readouts (ELISA) with transcript-level changes (qPCR) to strengthen conclusions. We can advise how to align sampling timepoints and normalization.

Yes. For biomarker trending, consistency and repeatability often matter more than a single absolute value. We recommend running the same control sample on every plate, keeping incubation times consistent, and using the same curve-fitting method throughout the project. If you plan cross-study comparisons, we can suggest a bridging strategy using shared controls to minimize lot-to-lot and day-to-day variation.

Edge effects are usually linked to temperature gradients, evaporation, and uneven timing. We suggest equilibrating reagents to room temperature, sealing plates during incubations, avoiding placing plates near vents, and using consistent multichannel pipetting patterns. If your lab environment is variable, consider reserving edge wells for blanks/controls. These steps typically reduce drift and improve replicate agreement.