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Macrophage IL-1 beta ELISA Kit, Colorimetric (MTS-1123-HM64)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for quantitative measurement of IL-1 beta (IL1B) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Analytical Method

Quantitative

Sample Type

Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Specificity

IL-1 beta (IL1B)

Specification

Size

96 tests

Sample Volume

100 μL

Assay Time

3 h

Plate

Pre-coated

Bioassay Target Name

IL-1 beta (IL1B)

Storage

4 °C, -20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

interleukin 1 beta

Synonyms

IL-1; IL1F2; IL1beta; IL1-BETA

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Similarly, IL-1B has been implicated in human osteoarthritis pathogenesis. Patients with severe Coronavirus Disease 2019 (COVID-19) present elevated levels of pro-inflammatory cytokines such as IL-1B in bronchial alveolar lavage fluid samples. The lung damage induced by the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL-1B. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM454	Human		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM455	Human		3.9-250 pg/mL	Inquiry
MTS-1123-HM456	Rat		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM457	Mouse		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM458	Rat		31.2-2000 pg/mL	Inquiry
MTS-1123-HM459	Mouse		12.5-800 pg/mL	Inquiry
MTS-1123-HM460	Rabbit		7.8 pg/mL-500 pg/mL	Inquiry
MTS-1123-HM461	Mouse		6.25 pg/mL-400 pg/mL	Inquiry
MTS-1123-HM462	Pig		1.56 pg/mL-100 pg/mL	Inquiry
MTS-1123-HM463	Human		125 pg/mL-8000 pg/mL	Inquiry
MTS-1123-HM464	Dog		7.8 pg/mL-500 pg/mL	Inquiry
MTS-1123-HM465	Rabbit		3.12 pg/mL-200 pg/mL	Inquiry
MTS-1123-HM466	Chicken		7.8 pg/mL-500 pg/mL	Inquiry
MTS-1123-HM467	Cow		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM468	Horse		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM469	Fish		3.12 pg/mL-200 pg/mL	Inquiry
MTS-1123-HM470	Goat		15.625 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM471	Dog		7.8 pg/mL-500 pg/mL	Inquiry
MTS-1123-HM472	Monkey		7.8 pg/mL-500 pg/mL	Inquiry
MTS-1123-HM473	Sheep		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM474	Rabbit		3.12 pg/mL-200 pg/mL	Inquiry
MTS-1123-HM475	Cow		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM476	Guinea Pig		7.8 pg/mL-500 pg/mL	Inquiry
MTS-1123-HM477	Golden Syrian Hamster		12.5 pg/mL-800 pg/mL	Inquiry
MTS-1123-HM478	Cat		16-4.000 pg/mL	Inquiry
MTS-1123-HM479	Hamster		12.5-800 pg/mL	Inquiry
MTS-1123-HM480	Rhesus Monkey		7.8 pg/mL-500 pg/mL	Inquiry

FAQs Customer Reviews Related Products

Is this IL-1β kit appropriate for detecting inflammasome-driven responses in macrophages?

Yes, IL-1β is a classic inflammasome-associated cytokine, and a colorimetric ELISA is commonly used to quantify secretion changes after priming and activation. For best results, ensure that your workflow distinguishes pro-IL-1β induction from mature IL-1β secretion, and collect supernatants at consistent time points. Including relevant controls (e.g., priming-only vs priming + activation) helps interpret biology correctly.

Can I compare IL-1β levels between different macrophage models (THP-1 vs primary cells)?

You can compare within each model confidently, and cross-model comparisons are feasible if you standardize key variables. Use the same culture conditions where possible, match cell counts, and normalize stimulation intensity. Since different models can secrete different baseline levels, we recommend expressing results as fold-change versus matched controls, plus running an internal reference sample across plates to anchor inter-run consistency.

My samples sometimes contain residual cell debris-will that affect the assay?

Debris can increase background and variability in any ELISA. We recommend centrifuging samples to remove particulates and transferring only the clear supernatant. If you must use crude samples, dilute them and consider running duplicates at two dilutions to confirm linearity. Clean handling and consistent washing steps usually solve most "noisy" IL-1β results without needing special equipment.

Helped confirm inflammasome activation with clear IL-1β secretion differences
We used the kit to verify IL-1β secretion after inflammasome activation and saw strong separation between conditions. The assay fit our standard ELISA workflow and didn't require unusual optimization. Our biggest improvement came from spinning down supernatants carefully and standardizing time points. Once we did that, replicates tightened up and the data aligned well with our phenotype readouts.
Straightforward cytokine ELISA that worked well for routine macrophage assays
This kit became part of our routine cytokine panel. It's easy for new team members to learn and provides consistent trends when you follow the wash steps carefully. We used it primarily for culture supernatants and occasionally for more complex matrices after dilution. For lab workflows that need dependable IL-1β tracking without complicated instrumentation, it performed as expected.
Good results after we implemented a quick dilution strategy for consistency
Our IL-1β secretion sometimes landed near the edge of our working range, so we started running two dilutions per sample. That simple change improved consistency and reduced reruns. The kit readouts matched our biological expectations and helped us decide which stimulation conditions to bring forward into deeper mechanistic experiments. Solid performance for the price and effort.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.