Macrophage Interleukin 23 (IL23) Competition ELISA Kit (MTS-1123-HM80)

This kit is designed for quantitative IL-23 measurement using a competition ELISA workflow, which is commonly chosen when the target is small or when sample conditions can challenge classic sandwich formats. To improve matrix compatibility, we recommend running a dilution linearity check (serially dilute your supernatant) and comparing recovery to the standard curve. Including a matrix-matched blank and spiked controls helps identify interference early and supports reliable interpretation.

For multi-plate projects, keep timing and temperature consistent across plates: equilibrate reagents to room temperature, use the same incubation durations, and wash thoroughly and uniformly. Use duplicate or triplicate wells, include a consistent internal control sample on every plate, and avoid repeated freeze-thaw cycles of standards and samples. If you expect values near the assay limits, pre-test a subset to choose the best dilution factor, then lock that dilution for the full study.

IL-23's biology can influence how you interpret results because different sample conditions may favor intact cytokine versus subunit presence. A competition ELISA typically reports the analyte signal based on competitive binding behavior, so consistent sample handling is important. If your study requires confidence around biologically active IL-23 versus related components, pair protein quantification with orthogonal readouts (e.g., pathway markers) and standardize sample collection and storage across conditions.