Macrophage Colony Stimulating Factor 1 (M-CSF/CSF1) ELISA Kit, qPCR (MTS-1123-HM60)

Yes, it's designed for macrophage-relevant CSF1 detection and works well with conditioned media when samples are clarified and handled consistently. To reduce false negatives, we recommend minimizing freeze-thaw cycles, using protease inhibitors if your workflow allows, and running a dilution series to ensure the signal falls within the assay's effective window. Including a positive control (stimulated macrophage supernatant) helps confirm matrix compatibility.

This kit format is intended to support qPCR-style readouts/compatibility in workflows that combine cytokine profiling with transcriptional validation. In practice, you are still assessing CSF1 in a standardized immunoassay setup, while using qPCR results as a complementary layer for pathway interpretation (e.g., CSF1 transcription vs secretion). Many labs pair it with macrophage polarization panels to connect protein output to upstream gene regulation.

Species coverage is typically reagent- and antibody-specific, so cross-species use is not automatically guaranteed. If you are measuring both mouse and human CSF1, we recommend selecting the corresponding species-reactive configuration to ensure accurate binding and calibration. If you want a single workflow across species, we can advise on parallel validation: run each matrix with appropriate standards and check dilution linearity to confirm performance.