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Macrophage Chemokine Ligand 11 (CXCL11) ELISA Kit, Colorimetric (MTS-1123-HM116)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for quantitative measurement of Chemokine Ligand 11 (CXCL11) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Analytical Method

Quantitative

Sample Type

Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Specificity

Chemokine Ligand 11 (CXCL11)

Specification

Size

96 tests

Sample Volume

100 μL

Assay Time

3 h

Plate

Pre-coated

Bioassay Target Name

Chemokine Ligand 11 (CXCL11)

Storage

4 °C, -20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

C-X-C motif chemokine ligand 11

Synonyms

IP9; H174; IP-9; b-R1; I-TAC; SCYB11; SCYB9B

Background

Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Chemokines are divided into 2 major subfamilies, CXC and CC. This antimicrobial gene is a CXC member of the chemokine superfamily. Its encoded protein induces a chemotactic response in activated T-cells and is the dominant ligand for CXC receptor-3. The gene encoding this protein contains 4 exons and at least three polyadenylation signals which might reflect cell-specific regulation of expression. IFN-gamma is a potent inducer of transcription of this gene. Two transcript variants encoding different isoforms have been found for this gene.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM779	Human		62.5 pg/mL-4000 pg/mL	Inquiry
MTS-1123-HM780	Mouse		31.2 pg/mL-2000 pg/mL	Inquiry
MTS-1123-HM781	Rat		46.88 pg/mL-3000 pg/mL	Inquiry
MTS-1123-HM782	Cow		46.88 pg/mL-3000 pg/mL	Inquiry

FAQs Customer Reviews Related Products

How do I decide between CXCL11 colorimetric quantification and a more "relative" approach? We have both mechanistic and screening needs.

Use the colorimetric quantitative kit when you need numeric concentrations to compare across studies, timepoints, or manuscripts, or when you need to set thresholds. Because this CXCL11 kit is positioned for quantitative measurement by colorimetric readout, it fits well for those applications. If you are doing early screens with many conditions where rank ordering is enough, a semi-quantitative approach can be faster. Many teams start with screening/relative profiling, then confirm key findings using a quantitative colorimetric ELISA for the figures that matter.

My macrophage activation cocktail includes components that can increase background. What practical steps help reduce nonspecific signal in a colorimetric ELISA?

Background in colorimetric ELISA usually comes from matrix effects (serum proteins, cell debris) or suboptimal washing/blocking. Clarify samples by centrifugation and consider diluting into the kit's recommended diluent to normalize matrix composition. Ensure wash steps are thorough-insufficient washing is a top cause of high background. If you suspect certain additives are interfering, run a spike-and-recovery check using a representative treated sample to see whether recovery drops, which indicates inhibition. Finally, keep timing consistent during substrate development; stopping reactions at uniform timepoints can significantly tighten replicate CVs.

I need publishable, quantitative CXCL11 data from macrophage supernatants. Does this kit support strict quantification, and what's the best approach for building confidence in the standard curve?

This product is described as a sandwich ELISA kit for quantitative measurement of CXCL11 with a colorimetric detection method. For publishable quantification, focus on standard curve discipline: run the full curve in duplicate (or triplicate if space allows), avoid edge effects by thoughtful plate layout, and keep incubation times consistent. If your sample signals cluster near the top or bottom of the curve, adjust dilution so they fall in the mid-range where precision is best. Also include a reference control sample across plates to monitor inter-plate variation.

Strong quantitative performance for CXCL11 in macrophage-conditioned media experiments
We needed absolute CXCL11 quantification for a macrophage chemotaxis project and selected this colorimetric sandwich ELISA because it's clearly framed for quantitative measurement. The standard curve behaved smoothly, and our samples fit well after a simple dilution optimization. What I liked most was that the assay produced believable numbers with reasonable replicate variation, making it suitable for reporting in our internal slides and drafts. We also ran the same control sample on multiple plates, and the results stayed within an acceptable window, which improved our confidence in inter-day comparability.
Easy to integrate into routine ELISA workflows with consistent readout
This kit dropped into our existing ELISA routine without requiring special instrumentation beyond a standard plate reader. The colorimetric signal was clear, and the quantitative framing helped us communicate results across teams that prefer concentration values rather than fold-change. We did have to be careful about washing (our first run had slightly elevated background), but once we tightened wash technique and clarified samples, the assay became very reproducible. It's a solid option when you want straightforward CXCL11 quantification from macrophage-related samples.
Good sensitivity for biological differences; supports dose-response interpretation well
We used the CXCL11 colorimetric ELISA for a dose-response experiment with inflammatory stimulation. The assay captured a clear trend with increasing stimulus, and the quantitative output made it easier to fit curves and compare potency between compounds. Because it's described as a sandwich ELISA for quantitative colorimetric detection, it aligned with our need for numeric reporting rather than relative ranking only. As usual, careful sample dilution mattered: once we placed samples in the mid-range of the curve, replicate precision improved and the data were easy to interpret.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.