Macrophage Interleukin 12 (IL12) Competition ELISA Kit (MTS-1123-HM77)

This IL‑12 kit is a colorimetric, quantitative competition ELISA supporting cell culture supernatant, plasma, serum, and tissue homogenate. Because it's labeled quantitative, you can obtain concentration estimates using a standard curve, but competition assays demand consistent technique. For screening, it also works well for ranking samples-just ensure your dilution scheme places samples in the responsive portion of the curve.

The stated assay time is 1.5 hours, and the sample volume is 100 µL per well. That relatively short runtime can be useful for busy schedules. To maintain quality, pre-plan your plate map, prepare standards and samples before starting, and keep timing synchronized across rows to avoid edge effects-especially important for competition curves where timing differences can shift signal.

Storage is listed at 4°C, with a 6-month expiry. For multi-run studies, keep reagents sealed, avoid contamination, and follow protocol guidance for any reconstituted components. If you will not use all wells at once, plan your experiments so standards and controls are repeated each run. This helps compare results across time and detect any gradual performance changes.