Macrophage Chemokine Ligand 16 (CCL16) ELISA Kit, qPCR (MTS-1123-HM98)

ELISA alone tells you how much CCL16 protein is present in the extracellular environment, but it may not reveal whether changes are driven by transcription, secretion, or protein stability. Adding qPCR gives you a second, mechanistically informative measurement that can confirm induction at the mRNA level or highlight post-transcriptional regulation. Reviewers often respond well to paired datasets because they strengthen causal interpretation and reduce the chance that a single-method artifact drives conclusions.

Yes, and that's a common use case. Early time points may show mRNA changes before protein accumulates, while later time points often reveal clearer secretion differences. We recommend selecting at least one early and one late time point, running matched ELISA and qPCR samples, and using consistent normalization rules. If you share your stimulation model and expected kinetics, we can suggest practical time points and sample handling to capture both phases effectively.

If volume is limited, prioritize ELISA on supernatants because secretion is often your primary functional endpoint, then perform qPCR on a subset of key conditions or time points to validate mechanism. Another strategy is to pool replicates for qPCR when the goal is trend confirmation rather than precise quantification. We can help you design a minimal yet defensible sampling plan that preserves interpretability while respecting sample constraints.