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Macrophage Chemokine Ligand 17 (CCL17) Competition ELISA Kit (MTS-1123-HM92)

Datasheet

Overview

Description

Creative Biolabs provides competition ELISA kit for quantitative measurement of Chemokine Ligand 17 (CCL17) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Competition ELISA

Analytical Method

Quantitative

Sample Type

Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Specificity

Chemokine Ligand 17 (CCL17)

Specification

Size

96 tests

Sample Volume

100 μL

Assay Time

1.5 h

Plate

Pre-coated

Bioassay Target Name

Chemokine Ligand 17 (CCL17)

Storage

4 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

C-C motif chemokine ligand 17

Synonyms

TARC; ABCD-2; SCYA17; A-152E5.3

Background

This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for T lymphocytes, but not monocytes or granulocytes. The product of this gene binds to chemokine receptors CCR4 and CCR8. This chemokine plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM684	Dog		250-5000 pg/mL	Inquiry
MTS-1123-HM685	Mouse		250-5000 pg/mL	Inquiry
MTS-1123-HM686	Rabbit		250-5000 pg/mL	Inquiry
MTS-1123-HM687	Monkey		25-500 pg/mL	Inquiry
MTS-1123-HM688	Human		25-500 pg/mL	Inquiry
MTS-1123-HM689	Guinea Pig		250-5000 pg/mL	Inquiry

FAQs Customer Reviews Related Products

What sample types can I use, and is this kit meant for quantitative measurement rather than just relative comparison?

This competition ELISA kit is designed for quantitative measurement of CCL17 and supports common matrices including cell culture supernatant, plasma, serum, and tissue homogenate. To get reliable absolute values, we recommend running samples in appropriate dilution series and including replicates. If you anticipate high background (e.g., tissue homogenates), we can advise on dilution and control setup to keep results within the working range.

How long does the assay take and what are the basic plate and handling requirements?

The kit is specified as a 96-test, pre-coated plate format with an assay time of about 1.5 hours, using 100 μL sample volume per well. Storage is listed as 4°C with a typical 6-month expiry, and protocol guidance is referenced in the kit documentation. These features make it suitable for routine quantification when you need a consistent, time-efficient workflow.

Which species/reactivity options exist, and how do I choose the right catalog variant for my model?

The product listing shows multiple sub-catalog variants with reactivity options including Dog, Mouse, Rabbit, Monkey, Human, and Guinea Pig, each with a defined sensitivity/detection range (for example, some variants list 25-500 pg/mL while others list 250-5000 pg/mL). Selection should be guided by your species and expected concentration range. If you tell us your model and predicted CCL17 levels, we can recommend the best-fit variant.

Fast competition ELISA workflow with clear quantitative behavior
We picked the competition format because we wanted a robust CCL17 quantification method across different sample types. The turnaround time was a major advantage; the assay fit comfortably into a short workflow and still delivered meaningful numbers. After validating a dilution series, we obtained consistent measurements and could compare treatment groups without ambiguity. Documentation and support were adequate for troubleshooting minor issues like unexpected high readings in one matrix.
Pre-coated plate convenience and predictable performance across plates
The pre-coated 96-test format simplified setup and reduced day-to-day variability. Once we standardized incubation timing and washing, replicate agreement improved and plate-to-plate drift was limited. We used pooled controls to track consistency across multiple experimental days, and that approach worked well with this kit. It's a good option when you need a repeatable CCL17 readout for macrophage stimulation experiments and want to keep the protocol efficient.
Useful for cross-sample comparisons after careful dilution optimization
We tested cell supernatants and a more complex matrix and found that the key to success was choosing the right dilution factor. The kit responded sensibly to dilution, which helped us confirm we were in a reliable measurement window. After that, trends matched biological expectations and correlated with other immune markers we tracked. Customer support also helped clarify best practices for controls and acceptance criteria, which improved our confidence in reporting.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.