Macrophage Migration Inhibitory Factor (MIF) Competition ELISA Kit (MTS-1123-HM50)

Yes. Competitive ELISA is often chosen when you expect a broad range of analyte concentrations. We recommend running a quick pilot dilution series (e.g., neat, 1:2, 1:5, 1:10) on your first batch so you can lock in a dilution factor for future runs. This reduces repeat assays, keeps signals within the working window, and improves between-plate comparability.

Matrix can influence binding and background in any immunoassay. For best confidence, we suggest spike-and-recovery and dilution linearity checks using your actual matrix: add known standard into serum/plasma/supernatant, then confirm recovery is consistent and that serial dilutions remain proportional. If you see suppression or elevated background, we can advise dilution buffers, sample dilution, or parallelism strategies to stabilize results.

Not necessarily. Specificity depends primarily on antibody quality and assay design. Competitive formats can be very specific, especially when the antibody epitope is well-characterized. Sandwich assays may provide strong signals because two antibodies bind different epitopes, but competitive assays can be advantageous for smaller targets or when only one high-performing antibody pair is available. We provide protocol guidance to optimize signal-to-noise.