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Macrophage Mannose Receptor 1 (MRC1) ELISA Kit, Colorimetric (MTS-1123-HM1)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for quantitative measurement of Mannose Receptor C Type 1 (MRC1) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Analytical Method

Quantitative

Sample Type

Cell Culture Supernatant, Cell Lysate, Plasma, Serum

Specificity

Mannose Receptor C Type 1 (MRC1)

Specification

Size

96 tests

Sample Volume

100 μL

Assay Time

3 h

Plate

Pre-coated

Bioassay Target Name

Mannose Receptor C Type 1 (MRC1)

Storage

4 °C, -20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

mannose receptor C-type 1

Synonyms

MMR; hMR; CD206; MRC1L1; CLEC13D; CLEC13DL; bA541I19.1

Background

The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM125	Rat		0.15 ng/mL-10 ng/mL	Inquiry
MTS-1123-HM126	Mouse		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM127	Human		0.15 ng/mL-10 ng/mL	Inquiry
MTS-1123-HM128	Human		0.41-100 ng/mL	Inquiry
MTS-1123-HM129	Human		0.312 ng/mL-20 ng/mL	Inquiry
MTS-1123-HM130	Sheep		3.12 ng/mL-200 ng/mL	Inquiry

FAQs Customer Reviews Related Products

I'm measuring CD206/MRC1 in macrophage lysates and some samples contain mild detergent. Will this kit tolerate common lysis buffers, and how should I minimize matrix effects?

This colorimetric sandwich ELISA is designed for quantitative measurement in multiple sample types, but buffer composition can influence binding and background. We recommend using a detergent-compatible lysis buffer at the lowest effective detergent concentration, clarifying lysates by centrifugation, and running a dilution series to confirm parallelism versus the standard curve. Including matrix-matched blanks and spike-recovery checks helps identify interference early and protects accuracy.

I need to compare MRC1 levels across donors and multiple plates. What steps do you suggest to reduce inter-plate variation and improve comparability?

For multi-plate studies, consistency is everything. Use the same incubation times, temperature, and plate-washing approach across all runs, and avoid changing pipette tips or reservoirs mid-plate to reduce drift. We strongly suggest including an internal control sample (a pooled lysate/supernatant) on every plate so you can normalize plate-to-plate shifts. Reading at the same time window after stopping the reaction will further stabilize the colorimetric signal.

If my MRC1 concentration is outside the standard curve, should I dilute and re-run, or can I extrapolate the curve?

We do not recommend extrapolating beyond the validated curve because colorimetric response may become non-linear at the extremes. The best practice is to dilute high samples into the assay's working range and re-run, or concentrate low samples (when feasible) while maintaining protein integrity. Running a quick pilot with 2-3 dilutions per sample can identify the optimal dilution factor and prevents wasted plates while keeping results defensible.

Reliable quantitative readout for CD206 across diverse macrophage samples
We used this MRC1 colorimetric kit to quantify CD206 changes during macrophage polarization. The workflow was straightforward and the signal-to-background was stable when we followed strict wash timing. Our key lesson was to test a small dilution series first; once we locked that down, replicate CVs were consistently acceptable and plate-to-plate trends matched biology. Support was responsive when we asked about buffer compatibility and recommended controls.
Good performance after optimizing sample dilution and wash consistency
Initial runs showed slightly elevated background in a subset of donor lysates, but after clarifying lysates and increasing dilution, the curve fit improved and recovery looked reasonable. The kit is convenient for screening lots of samples, and the colorimetric readout works well on standard plate readers. I'd recommend including a pooled reference sample on each plate for normalization if you're running a large study.
Solid kit for routine MRC1 quantification with practical troubleshooting
We needed a dependable ELISA for MRC1 in macrophage-conditioned media and lysates. The assay was easy to implement with standard lab equipment, and the instructions aligned with typical sandwich ELISA steps. The biggest improvement came from careful pipetting and consistent incubation timing; once standardized, our biological replicates separated cleanly. Shipping and packaging were fine, and the reagents performed as expected.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.