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Macrophage Interleukin 12 (IL12) ELISA Kit, qPCR (MTS-1123-HM78)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for semi-quantitative measurement of Interleukin 12 (IL12) in different sample types by qPCR.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

qPCR

Method Type

Sandwich ELISA

Analytical Method

Semi-Quantitative

Sample Type

Cell Culture Supernatant, Plasma, Serum

Specificity

Interleukin 12 (IL12)

Specification

Size

96 tests

Sample Volume

25 µL

Plate

Pre-coated

Bioassay Target Name

Interleukin 12 (IL12)

Storage

4 °C, -20 °C, -80 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

interleukin 12

Synonyms

IL-12

Background

Interleukin 12 (IL-12) is an interleukin that is naturally produced by dendritic cells, macrophages, neutrophils, and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 belongs to the family of interleukin-12. IL-12 family is unique in comprising the only heterodimeric cytokines, which includes IL-12, IL-23, IL-27 and IL-35.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM879	Mouse	0.5 pg/mL		Inquiry
MTS-1123-HM880	Human	0.1 pg/mL		Inquiry
MTS-1123-HM881	Rhesus Monkey	4 pg/mL		Inquiry
MTS-1123-HM882	Pig	0.22 ng/mL		Inquiry
MTS-1123-HM883	Sheep	User optimized		Inquiry

FAQs Customer Reviews Related Products

What is the main use case for an IL‑12 ELISA kit with qPCR readout?

qPCR-detection ELISA formats are typically used when you want an alternative readout modality and are comfortable reporting semi‑quantitative outcomes such as relative changes between conditions. We recommend it for comparative profiling, time-course studies, and screening where rank order and fold-change are the key outputs.

How should we validate semi‑quantitative IL‑12 results for internal decision-making?

A practical validation plan includes: (1) a dilution series of representative samples to confirm monotonic behavior; (2) repeated internal reference controls across plates; and (3) correlation checks against an orthogonal method (e.g., colorimetric quantitative ELISA) on a subset. While semi‑quantitative outputs are powerful for comparisons, documenting repeatability and control stability strengthens confidence in decisions derived from the data.

Does IL‑12 biology suggest any special handling of stimulated macrophage samples before testing?

IL‑12 is produced by immune cells including macrophages in response to antigenic stimulation, so stimulated samples can vary widely and may include interfering factors. We recommend clarifying supernatants, avoiding repeated freeze-thaw, and performing a pilot dilution scan to place samples within a responsive detection window. For time-course studies, freeze all samples promptly and test them in the same batch when possible.

Helpful semi‑quantitative IL‑12 kit for ranking stimulation conditions quickly
We used this kit primarily to rank multiple macrophage stimulation conditions and time points. Because we only needed relative differences, a semi‑quantitative approach was fine and gave clear trends that matched IL‑12 biology. With good internal controls, the run-to-run pattern was consistent. It helped us narrow conditions for follow-up with a quantitative ELISA, saving time and reagents.
Good complement to quantitative ELISA for low-signal or screening phases
During early screening, our goal was to see which treatments increased IL‑12 most strongly. This semi‑quantitative style kit fit that need and aligned with what we expect from macrophage activation biology. After identifying top conditions, we validated a subset using a standard quantitative assay. The combination improved our workflow efficiency and kept decision-making evidence-based.
Consistent IL‑12 trend detection across repeated macrophage stimulation batches
We repeated stimulation experiments over several weeks. As long as we used a consistent reference control and standardized sample handling, we saw stable relative IL‑12 trends that tracked with stimulation strength. This supported our internal go/no-go choices before investing in deeper profiling. It's best for comparative insights, and it performed reliably when treated that way.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.