Macrophage Chemokine Ligand 18 (CCL18) Competition ELISA Kit (MTS-1123-HM35)

This product is a competition ELISA with colorimetric detection for quantitative measurement of CCL18. Compared with sandwich ELISAs, competitive formats can be selected when you prefer that assay design or when you want an alternative workflow. The key operational difference is that competitive assays often show an inverse signal relationship, so careful standard curve review is essential. Many customers choose it as a complementary method for cross-checking results.

The kit lists cell culture supernatant, plasma, serum, and tissue homogenate as sample types, with an assay time of ~1.5 hours and 100 μL sample volume. The plate is specified as strips (12 × 8), which can be convenient if you don't want to run a full plate at once. This shorter runtime is helpful for fast turnaround studies or when you need same-day results.

The most common pitfalls are (1) forgetting the inverse relationship between signal and analyte concentration, and (2) selecting a dilution that places samples on a flat part of the curve. We recommend running at least two dilutions initially and confirming that your control behaves as expected. Plot the standards immediately, verify curve direction, and keep timing consistent. Those steps prevent misreads and help maintain quantitative reliability across plates.