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Macrophage Chemokine Ligand 5 (CCL5) ELISA Kit, qPCR (MTS-1123-HM87)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for semi-quantitative measurement of Chemokine Ligand 5 (CCL5) in different sample types by qPCR.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

qPCR

Method Type

Sandwich ELISA

Analytical Method

Semi-Quantitative

Sample Type

Cell Culture Supernatant, Plasma, Serum

Specificity

Chemokine Ligand 5 (CCL5)

Specification

Size

96 tests

Sample Volume

25 µL

Plate

Pre-coated

Bioassay Target Name

Chemokine Ligand 5 (CCL5)

Storage

4 °C, -20 °C, -80 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

C-C motif chemokine ligand 5

Synonyms

SISd; eoCP; SCYA5; RANTES; TCP228; D17S136E; SIS-delta

Background

This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM895	Mouse	0.1 pg/mL		Inquiry
MTS-1123-HM896	Rat	0.4 pg/mL		Inquiry
MTS-1123-HM897	Human	0.3 pg/mL		Inquiry
MTS-1123-HM898	Pig	4.69 pg/mL		Inquiry
MTS-1123-HM899	Cat	0.07 pg/mL		Inquiry

FAQs Customer Reviews Related Products

What should I know about sample volume and plate setup before ordering?

The kit is specified as 96 tests with a pre-coated plate and 25 µL sample volume per well. It's described as a sandwich ELISA with qPCR detection and semi-quantitative analysis, and sample types include cell culture supernatant, plasma, and serum. These specs are useful for planning when sample volume is limited.

How should I interpret "semi-quantitative" CCL5 results for macrophage activation experiments?

In many macrophage activation studies, what matters most is the direction and magnitude of change across conditions, not a single absolute concentration claim. Semi-quantitative kits can be excellent for that use case when you keep handling consistent and include appropriate controls. If your downstream requirement is absolute quantitation, consider confirming key findings with a fully quantitative colorimetric method on a subset of samples.

What storage approach minimizes kit degradation across a long study?

Storage is listed across 4°C, -20°C, and -80°C depending on components, which helps long studies if you follow the protocol's guidance on which reagents belong where. Split sensitive reagents into aliquots if allowed, avoid repeated freeze-thaw cycles, and track open dates carefully. Keeping consistent thawing practices across run days also improves reproducibility and reduces drift.

Great option when sample volume is limited and trends matter most
We often had limited macrophage supernatant volume, and the 25 µL requirement made a big difference. The kit gave consistent relative changes across our treatment groups and aligned well with our transcriptional markers. We treated it as a semi-quantitative method and designed our stats accordingly. With internal controls per plate, we achieved reproducible results across multiple weeks.
Pre-coated plates improved consistency between operators in our lab
Different users ran the assay in our lab, and pre-coated plates helped reduce variability. We standardized incubation timing and washing technique, and the results were stable across runs. This kit is particularly good for comparative studies-screening conditions, ranking stimuli, or validating pathway effects-where you want dependable directionality without spending excessive sample volume.
Strong fit for macrophage studies with well-controlled sample handling
Once we controlled collection timepoints and avoided freeze-thaw cycles, the assay produced clean separation of groups and good reproducibility. The storage flexibility helped us manage reagents across the project. We also liked that the method naturally encouraged a disciplined workflow with controls and consistent processing. Overall, it supported our CCL5 profiling goals effectively.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.