Macrophage Chemokine Ligand 24 (CCL24) ELISA Kit, Colorimetric (MTS-1123-HM94)

Colorimetric ELISA is widely used for macrophage supernatants because it balances sensitivity with operational simplicity. For moderate changes, the most important factor is keeping your samples within the linear portion of the standard curve through proper dilution. We recommend an initial pilot plate to determine the best dilution factor and to check recovery using spiked controls. If you provide expected biology and sample matrix, we can suggest a realistic starting dilution plan.

A cost-effective plan is to run technical duplicates for each sample and include a shared reference control in duplicates across the plate, then prioritize biological replicates at the experimental design level. This reduces spending on repeated technical wells while keeping statistical power where it matters most. We can also help you design a plate map that minimizes edge effects and keeps standard curve placement consistent, which further improves comparability across groups.

Standardize timing, use calibrated multichannel pipettes, and keep wash steps consistent-these three actions typically reduce most variability. Preparing a master mix when possible, labeling reagents clearly, and using a checklist for each plate also prevent common mistakes. If you're running multiple plates, including an identical control sample on each plate provides a simple way to monitor drift. Our support team can review your throughput plan and suggest efficiency improvements.